Introduction: Background : Cancer has affected a lot of people and it is the second leading cause of death in the world after heart diseases for many years .And it is vascular. Cancer is a very complex genetic, epigenetic, and environmental disease with great diversity in tissue, tumor, and cellular levels. Scientists are trying to propose the best option for each patient's treatment regimen by accurately identifying the mutations involved in the development of each cancer, in order to prevent the side effects of chemotherapy as much as possible with this approach and to maximize the efficiency of the treatment RICTOR overexpression was associated with increase of tumor size, lymph node metastasis, and advanced TNM stage with poorer overall survival. RICTOR attenuates cell cycle progression and increases of apoptosis. Previous research has identified RICTOR as an important mediator of tumor progression and metastasis. identified that mTOR is a serine-threonine protein kinase that belongs to the family of phosphoinositide kinase-3 (PI3K) related kinases and plays an essential role in cell metabolism, growth and survival. Based on its sensitivity to rapamycin, mTOR was classified into two structurally and functionally distinct multiprotein complexes called mTOR complex1(mTOR1) and mTOR complex2(mTOR2) Extensive research has shown that mTORC plays an important role in the PI3K-AKT pathway, promoting cell metabolism, growth, and survival. RICTOR is a component of the mTORC2 complex that determines the stability and integrity of the mTORC2 complex. Many studies have shown that mTOR overexpression is common in gastric cancer, and p-mTOR is suggested as an independent prognostic factor for gastric cancer. As early as 1984 , the first generation of mTOR inhibitors, rapamycin, was used for tumor treatment subsequent reports showed that the combination of rapamycin and other anti-tumor drugs had a very good effect.
Methods: First, gastric cancer tissue samples were collected from patients by a specialist doctor, and then RNA is extracted from the tissue. After collecting samples, RNA extraction and cDNA synthesis we effectuate. All work steps are done under the laminar hood.
Results: After some researches, there was a significant increase of the expression of Rictor gene in cancer samples compared to the samples of healthy people.
Conclusion: Considering that the effect of this gene on gastric cancer has increased, it can act as a biomarker for the prevention of gastric cancer.