• Green Synthesis of Copper nanoparticles Derived by Green Tea Extract and Determination of Antimicrobial Effects on Staphylococcus auroras and Escherichia coli
  • Nader Kazemi,1,* Sahar Rasouli,2 Mahdi Arfaei,3
    1. Nanobiotechnology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
    2. Department of Biology, Faculty of Basic Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
    3. Department of Biology, Faculty of Basic Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.


  • Introduction: In this study, the aqueous extract of green tea plant was used to produce copper nanoparticles and its antimicrobial activity was investigated. To identify the synthesized nanoparticles, different analyses, techniques and tools were used, including and electron microscope, T.E.M imaging was performed and the size of the nanoparticles was determined.
  • Methods: method that was used is the XRD and Zeta potential methods, which according to the given graphs, each graph has alternating peaks, which shows the certainty of the synthesis. The antimicrobial activity of copper nanoparticles synthesized using the micro dilution method was used for bacteria in Mueller Hinton culture medium, which was used according to the CLSI standard. In this method, eight wells started with a concentration of 250 micrograms per milliliter and progressed half by half until well eight.
  • Results: In the extraction process, first 19g of green tea leaves and its ingredients were prepared and milled then it was soaked in 190 ml of distilled water, stirred well and placed in the refrigerator for 48 hours. Afterb48 hours 4 milliliters of the extract were added to one-tenth molar copper oxide to a volume of 25 milliliters, and it was placed on a heater shaker at a temperature of 80 degrees Celsius for one hour, after adding the extract to the colored copper oxide solution changed from black to light brown. Synthesized nanoparticles showed activity against Staphylococcus auroras and Escherichia coli bacteria.
  • Conclusion: The results showed that the aqueous extract of green tea plants acts as a rejuvenating and stabilizing agent. Synthesized copper nanoparticles showed activity against both gram-positive (Staphylococcus auroras) and gram-negative (Escherichia coli) bacteria.
  • Keywords: antimicrobial, copper, green tea, nanoparticles.