The effects of Zinc Oxide Nanoparticles on Taxol induced testicular toxicity in mice
The effects of Zinc Oxide Nanoparticles on Taxol induced testicular toxicity in mice
zahra yengi maleki,1,*Monireh Mahmoodi,2
1. 1Department of Biology, Faculty of Sciences, Arak University, Arak, Iran. 2. 1Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
Introduction: The treatment of childhood cancer with chemotherapy drugs can result in infertility in adulthood. Taxol is the brand name of Paclitaxel, is a chemotherapeutic agent able to generate reactive oxygen species (ROS) and causes male infertility. In this study, we investigated the protective role of zinc oxide nanoparticles against the destructive effects of Taxol on testicular tissue in adult male NMRI mice.
Methods: 24 adult male NMRI mice were divided into four groups: control, taxol (5mg/kg), zinc nanoparticles (5mg/kg) and taxol + zinc nanoparticles and treated for 35 days Intraperitoneally. At the end of treatment, after anesthetizing the mice, body and testis weight and tissue parameters were evaluated with stereological technique. Data were analyzed by one-way analysis of variance and Tukey test.
Results: A significant decrease was observed in the testicular weight, the volume of testicular tissue, volume, height and diameter of seminiferous tubules, spermatogenesis indices and the number of germ cells in the Taxol group compared to the control group (p<0.001), while the interstitial tissue volume showed a significant increase in the Taxol group compared to the control group (p<0.05). The simultaneous treatment of zinc oxide nanoparticles with taxol significantly reduced the mentioned parameters compared to the Taxol group.
Conclusion: These findings suggested an antioxidant potential role for Zinc oxide nanoparticles in the protection of Taxol -induced testicular toxicities.