• Investigating the effect of lncRNA H19 on CST2 and GJB2 expression in breast cancer
  • Masoumeh Shojaei Barjouei,1,* Nasibeh Piroozram,2 Azizeh Asadzadeh,3
    1. MSc of biochemistry,Department of biology,faculty of science,Nour Danesh institute of higher education,Meymeh,Isfahan,Iran
    2. MSc of genetics, Department of Molecular Biology, Faculty of Basic Science, Islamic Azad University , Ahar , Tabriz , Iran
    3. Department of Biology, Faculty of Basic Sciences, Nourdanesh Institute of Higher Education, Meymeh, Isfahan, Iran


  • Introduction: Breast cancer is the most commonly occurring cancer in women. Long noncoding RNAs (lncRNAs) are noncoding transcripts with more than 200 nucleotides such as antisense RNAs, enhancer RNAs, and endogenous RNAs. lncRNAs can help as a tumor suppressor and oncogenes to cell growth, metabolism, and metastasis. Long non-coding RNA H19 is abnormally expressed in some types of cancers. Previous studies have shown the effects of H19 on breast cancer development, metastasis, and progress. Changes in the expression of CST2 and GJB2 genes have been reported in the early stages of tumorigenesis so that these two genes can be used as diagnostic markers. Therefore, the aim of this study is to investigate the expression changes of CST2, and GJB2 in patients with breast cancer and the effect of long non-coding RNA H19 on CST2, and GJB2 expression levels by bioinformatics approaches.
  • Methods: in this study, we used the GEO website (https://www.ncbi.nlm.nih.gov/gds) to find access codes related to Breast cancer. Also, to quickly access we used filters including Homo sapiens, series, and expression profiling by the array. The differentially expressed genes (DEGs) were identified using the GEO2R tool. Finally, investigating the effect of lncRNA H19 on CST2 and GJB2 expression was studied by lncRRisearch.
  • Results: We used GSE42568 for Breast Cancer Gene Expression Analysis, 104 breast cancer biopsies were studied in this microarray gene expression datasets. 11 samples with grade 1; 40 samples with grade 2; 53 samples with grade 3, and 17 normal breast tissues were assayed in GSE42568. The GEO2R tool (cut-off value: 1 and p-value < 0.05) showed that GJB2 with Logfc: -2.89 and CST2 with Logfc: -1.54 were down-regulated genes. In lncRRisearch results, the energy interaction of GJB2-H19 and CST2- H19 was -16.66 kcal/mol and -19.97 kcal/mol, respectively.
  • Conclusion: Our insilico study showed that H19 is involved in changing the gene expression profile of GJB2 and CST2.
  • Keywords: lncRNA, Breast cancer, GJB2, CST2, H19