Antimicrobial activity of copper nanoparticles derived by ajowan extract on Staphylococcus aureus and Escherichia coli
Antimicrobial activity of copper nanoparticles derived by ajowan extract on Staphylococcus aureus and Escherichia coli
Nader Kazemi,1,*Melika Sadeghi,2Mahdi Arfaei,3
1. Nanobiotechnology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran. 2. Department of Biology, Faculty of Basic Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran. 3. Department of Biology, Faculty of Basic Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Introduction: In this study, the aqueous extract of Trachyspermum copticum seeds were used to synthesis copper nanoparticles and its antimicrobial activity was investigated.To identify the synthesized nanoparticles, different analyses, techniques and tools were used, including T.E.M imaging was performed and the size of the nanoparticles was determined. Other methods that were used is the XRD and Zeta potential methods, which according to the given graphs, each graph has alternating peaks, which shows the certainty of the synthesis.
Methods: The antimicrobial activity of copper nanoparticles synthesized using the micro dilution method was used for bacteria in Mueller Hinton culture medium, which was used e cording to the CLSI standard. In this method, eight wells started by 250 mg/ml and progressed half by half until well eight.
In the extraction process, first about 19 grams of Trachyspermum copticum seeds were soaked in 190 ml of distilled water and storage in the refrigerator for 48 hours. After 48 hours 4 ml of the extract were added to one-tenth molar copper (II) oxide to a volume of 25 ml, and it was placed on a heater shaker at a temperature of 80oc for 1 hour, after adding the extract to the copper oxide solution changed from black to light brown.
Results: Synthesized nanoparticles showed activity against Staphylococcus auroras and Escherichia coli bacteria. Based on these data, copper nanoparticles derived by green method are the best choice for antimicrobial treatment.
Conclusion: The results showed that the aqueous extract of Trachyspermum copticum seeds acts as a rejuvenating and stabilizing agent. Synthesized copper nanoparticles showed activity against both Gram- positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria.