• Hesperidin suppressed Tumor Growth and increased cell survival on Induced Breast Cancer in BALB/c Mice
  • Mohsen rashidi,1,* Reihane khorasanian,2 Fatah rohani,3
    1. Department of Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. 2The Health of Plant and Livestock Products Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
    2. Department of Medical Genetics and Molecular Biology, Faculty of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran
    3. Faculty of Veterinary medicine of Shahrekord,Shahrekord,Iran


  • Introduction: Introduction: Hesperidin, a flavonoid, have been shown various biological effects including anticancer activity. The objective of the study was to investigate the protective effects of hesperidin against experimentally induced breast cancer in BALB/c.
  • Methods: Methods: In this study, we treated 4T1 tumor-bearing Balb/c mice with intraperitoneal injection of Hesperidin, Hesperidin +Doxorubicin, and saline as control groups. Body weight and tumor volume were measured before and after treatment. Hematoxylin and eosin (H & E) staining and immunohistochemistry of Ki-67, MMP2, MMP9, VEGF and E-cadherin markers were used as markers of proliferation. The effect of hesperidin on changes in tumor growth and survival rate was also investigated
  • Results: Results: The average tumor volume, average pCR and the percentage of destroyed tumor cells were lower in the hesperidin group with different doses than the normal saline group (P ≥ 0.05) and the percen survival was significantly higher (P ≥ 0.05). Also, the expression of Ki-67, VEGF, MMP2, MMP9, E-cadherin markers in the hesperidin group with different doses was significantly lower than the normal saline group. The expression of these markers in the hesperidin + doxorubicin group (10+20 mg) was lower than the normal saline group and the doxorubicin 10 mg group (P ≥ 0.05).
  • Conclusion: Conclusion: The results of this study confirm that hesperidin can be considered as a potential suitable treatment both in the presence of standard treatment (doxorubicin) and without it. The role of hesperidin as an inhibitor of tumor growth and increased cell survival was observed in this study. Therefore, it is suggested to design and conduct corresponding clinical studies to confirm the effect of hesperidin on breast cancer in humans.
  • Keywords: Keywords: Angiogenesis, Phatology, Breast cancer, Hesperidin