مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Quantitative analysis of biochemical characteristics and anti-cancer properties in MCF-7 breast cancer cell line: a comparative study between Ziziphus jujube honey and commercial honey
Quantitative analysis of biochemical characteristics and anti-cancer properties in MCF-7 breast cancer cell line: a comparative study between Ziziphus jujube honey and commercial honey
Samira Karbasi,1Amir Hassan Asadian,2Ehsaneh Azaryan,3Mohsen Naseri,4Asghar Zarban,5,*
1. Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran 2. Department of Horticultural Science, Faculty of Agriculture, University of Birjand, Birjand, Iran 3. Cellular and Molecular Research Center, Molecular Medicine Department, Birjand University of Medical Sciences, Birjand, Iran 4. Cellular and Molecular Research Center, Molecular Medicine Department, Birjand University of Medical Sciences, Birjand, Iran 5. Clinical Biochemistry Department, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
Introduction: There is increasing evidence that honey has anti-inflammatory, antioxidant, and anti-cancer effects. This study
aims to assess and contrast the cytotoxic, anti-metastatic, and apoptotic effects of Ziziphus jujube honey and commercial
honey on MCF7 cells.
Methods: Two honey samples, Ziziphus jujube (JH) and commercial honey (CH), were categorized into high and
low groups based on their phenolic content, antioxidant capacity, and diastase activity (PAD score). The viability and migration ability of MCF-7 cells treated with JH and CH were evaluated. Also, quantitative polymerase chain reaction (Q-PCR)
was performed to assess the effect of the two honey samples on the expression of Bax, p53, p21 and Bcl-2 genes.
Results: JH had a
total phenolic content of 606.4±0.1 µg gallic acid equivalent/mg, while CH had a value of 112.1±0.09 µg gallic acid equivalent/mg. The total antioxidant capacity of the two samples was compared. It was 203.5±10.5µM/l in JH and 4.6±10.5
µM/l in CH. In addition, JH had a diastatic activity of 524.1±0.25 U/l, while CH had a value of 209.7±0.56 U/l. According
to the results, JH had a high PAD value, while CH had a low PAD value. Cell viability was measured using the results of
the MTT assay. The results showed that JH inhibited the growth of MCF-7 cells more strongly (IC50 of 170±4.2 µg/ml)
than CH (IC50 of 385.3±4.5 µg/l). The scratch assay showed that treatment with JH decreased the migration rate of MCF-7
cells in a dose-dependent manner compared to the CH and control groups. In addition, the results of q-PCR analysis showed
significant upregulation of Bax, p53 and p21 genes and downregulation of Bcl-2 gene in the JH-treated group compared to
the CH and control groups.
Conclusion: These results showed that honey with an increased content of phenolic compounds, antioxidant capacity, and
diastatic activity has anticancer properties by effectively suppressing tumor development. This suppression occurs via several mechanisms, including suppression of proliferation and metastasis, and promotion of apoptosis.