Introduction: Background and purpose:
Staphylococcus aureus is a significant pathogen globally, causing acquired infections. The escalating resistance of this bacterium to antibiotics is a growing concern, with decreasing efficacy of current treatment options. This study seeks to explore and characterize antibiotic resistance genes in MRSA strains, aiming to elucidate the mechanisms underlying antibiotic resistance through an analysis of biological systems and gene ontology.
Methods: Materials and methods:
In this study, gene expression profiles associated with Staphylococcus aureus were analyzed. Subsequently, employing the WGCNA software package, co-expression networks of genes were constructed to identify main gene clusters and inter-gene relationships. Following this, the principal genes within each module were identified, and their functional attributes were scrutinized to elucidate their roles and relationships within the network. This comprehensive analysis aimed to investigate the interplay between these genes and their impact on the regulatory mechanisms underlying this biological process.
Results: Results:
The enrichment analysis of genes, pathways, and biological processes related to this bacterial resistance is conducted using specific modules. Subsequently, the most critical genes within each module are identified and presented as potential new candidates. In the following stage, computational tools are utilized to ascertain the regulatory roles of genes within the networks. Noteworthy genes such as qoxB, qoxA, qoxC, and qoxD associated with oxidoreductase enzyme activities, along with HslV, PotA, yidC, copA, and fba, are known as the main elements of this phenomenon.
Conclusion: Conclusion:
The results of the current study demonstrate that the systemic biology approach yields promising outcomes in analyzing the survival rates of patients afflicted with bacterial infections. Through this research, pivotal genes have been discerned which may serve as valuable prognostic biomarkers for overall survival, warranting further investigation in laboratory settings.