مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Biochemical identification of oral bacterial flora and their antibiotic susceptibilities in Macrovipera lebetina Snake
Biochemical identification of oral bacterial flora and their antibiotic susceptibilities in Macrovipera lebetina Snake
Rasoul Karamiani,1,*Khosrow Chehri,2Mozhgan Fatahi Dehpahni,3
1. Department of Biology, Faculty of Sciences, Razi University 2. Department of Biology, Faculty of Sciences, Razi University, 3. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences
Introduction: Infections are a common clinical complication after a snake bite. Knowledge about different microorganisms in the mouth of snakes is an important role in prescribing antibiotherapy for the snake bite. The aims of this research were to determine the cultivable of mouth bacteria of Macrovipera lebetina and their antibiotics sensitivity.
Methods: An oral cavity swab from a healthy adult Macrovipera was taken and cultured in aerobic conditions to identify microflora which present in the snake mouth. Isolated colonies were recognized using morphological and biochemical methods. All these colonies were tested for antibiotic sensitivity by the disk diffusion method.
Results: A total of nine Gram-negative bacteria were identified from the oral sample of the snake including Escherichia coli, Salmonella, Proteus, Pseudomonas, Klebsiella, Edwardsiella, Acinetobacter, Citrobacter, and Bordetella. A total of five Gram-positive bacteria were obtained such as Coagulase-negative Staphylococcus, S. aureus, Bacillus, Micrococcus, and Corynebacterium. Among tested antibiotics, all Gram- negative bacteria were completely resistant to Ceftriaxone and all Gram- positive bacteria were completely resistant to Penicillin. Some Gram- negative strains showed high susceptibility to Ciprofloxacin and Nitrofurantoin antibiotics, and some of the Gram- positive isolates represented high susceptibility to Clindamycin, Trimethoprim/sulfamethoxazole, Gentamycin, Ceftriaxone, Chloramphenicol, and Tetracycline.
Conclusion: The results of our study demonstrated noticeable microbial pathogens in the mouth cavity of Macrovipera lebetina. Therefore, in snakebite problems not only anti-venom therapy is needed but also antibiotic therapy should be checked in victims.