مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Investigating the relationship between follicle stimulating hormone and ovarian response in infertile women after IVF
Investigating the relationship between follicle stimulating hormone and ovarian response in infertile women after IVF
Khadijeh Afshoun,1,*Mohammadreza Pourmohammad,2Jina Khayatzadeh,3Parvin Torabzadeh,4
1. Clinical Research Development Unit, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran 2. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran 3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran 4. Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran
Introduction: Infertility and low fertility rate in the ectopic fertilization cycle can be due to the condition and level of hormonal levels caused by ovulation stimulation methods, which in cases of reduced ovarian reserve, the follicular response to gonadotropin stimulation is also reduced and few eggs will be produced. The present study aimed to investigate the predictive effect of follicle stimulating hormone (FSH) and number of antral follicles in infertile women before IVF on ovarian response and pregnancy success rate.
Methods: This cross-sectional study (descriptive-analytical) was conducted in 2020-2022 on 80 infertile women treated with ART infertility treatment, referring to the infertility center of Razavi Hospital in Mashhad. First, the levels of AMH, FSH, CBC, LFT, TFT, Ur, Cr, and Prolactin were measured and vaginal ultrasound was done to determine the number of antral follicles (AFC) and the number of gonadotropin ampoules needed was determined and recorded in the file. On the 20th to 21st day of the cycle, the patients were subjected to imitation transfer and GnRH ampoules were injected. By visiting again on the second day of menstruation, gonadotropin was injected. From the 6th day of injection onwards, ultrasound was performed every other day to see at least 2 follicles of 17 to 18 mm. 5 to 10 thousand hCG units were injected depending on the number of follicles, and then 36 to 40 hours later, ovulation was first performed and then for IVF patients. Data analysis was done using SPSS version 26 statistical software and chi-square tests, Fisher's exact test, one-way analysis of variance and Pearson correlation. A p value of less than 0.05 was considered significant.
Results: According to the ultrasound performed, the average number of antral follicles (follicles less than 10 mm) before treatment was 4.43±4.17. On average, the number of oocytes after treatment in patients was 3.09±3.17. The average HCG hormone at the end of treatment and 16 days after embryo transfer was 94.79±265.86 pmol/dL. FSH was 6.81±4.54 pmol/dL.
Conclusion: There was no difference in the amount of these hormones in women with positive and negative ovarian response, but there was a significant difference in pregnancy and pregnancy with positive outcome.