• Effect of SKEO on inflammatory genes using real-time polymerase chain reaction.
  • Gholamreza Shahsavari,1 Hamed Esmaeil Lashgarian,2 Masumeh Jalalvand,3 leila Abkhooie,4 Amirmasoud Jalalvand,5 Ghasem Mosayebi,6,*
    1. Department of Biochemistry, School of Medicine, Lorestan University of Medical Sciences,
    2. Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences
    3. Assistant Professor, Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences
    4. Assistant Professor, Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences
    5. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences
    6. Professor of Medical Immunology Department of Microbiology and Immunology, School of Medicine Molecular and Medicine Research Center Arak University of Medical Sciences


  • Introduction: Essential Satureja Khuzestanica oil (SKEO) and the monoterpene Carvacrol have anti-inflammatory properties, albeit without specifying the precise mechanism of its efficacy. Prostaglandin is one of the main mediators of inflammation regulated by the cyclooxygenase gene and nitric oxide synthase gene. This study examined the effects of SKEO and Carvacrol on the expression of the cyclooxygenase and nitric oxide synthase gene in the LPS-stimulated cell line.
  • Methods: Fresh parts of the plant were processed to prepare SKEO. Different doses of SKEO and Carvacrol were then used to treat an LPS-stimulated cell line. Following RNA extraction, gene expression analysis was conducted using real-time polymerase chain reaction.
  • Results: Results: In the LPS-stimulated macrophage cell line, essential S. Khuzestanica oil significantly reduced cyclooxygenase and nitric oxide synthase gene expression in a dose-dependent manner. Carvacrol had a lesser effect. Notably, essential S. Khuzestanica oil exhibited a significantly stronger inhibitory effect on cyclooxygenase and nitric oxide synthase gene expression than Carvacrol.
  • Conclusion: The decrease in cyclooxygenase and nitric oxide synthase gene expression indicates that S. Khuzestanica oil could be beneficial for managing inflammation-related conditions. Future research should focus on optimizing the dosage and formulation of SKEO to enhance efficacy and reduce side effects. These encouraging results emphasize the necessity of integrating herbal remedies into conventional treatment regimens, paving the way for innovative anti-inflammatory strategies.
  • Keywords: Essential oil, gene expression, inflammation, SKEO, LPS-stimulated macrophage