• Role of biofilm formation in antibiotic resistance of isolated Pseudomonas from clinical Samples
  • Forugh Faridi,1 Fatemeh Habibi,2 Nima Bahador,3,*
    1. Department of Microbiology College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran
    2. Department of Microbiology College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran
    3. Department of Microbiology College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran


  • Introduction: Pseudomonas aureoginosa is a gram negative bacterium with different virulence determinants. The organism grow at diverse range of temperature and has ability to tolerate most of habitats. Although the organism has some kinds of genes which is protect it from harsh environments, biofilm formation ability of the isolates can help them to protect them from some kinds of antibiotics. Therefore, the present study tried to evaluate correlation between biofilm formation and antibiotic resistance
  • Methods: In this study 459 clinical samples have been collected from laboratory of Shahid Mohammadi Hospital in Bandarabas. The samples were evaluated for presence of Pseudomonas and confirmed by biochemical as well as molecular technique using gyr gene. Then effect of some antibiotics using CLSI 2019 was evaluated on the isolates and biofilm formation was assessed using microplate techniques
  • Results: The results indicated that out of 459 samples 151 were confirmed as Pseudomonas using biochemical and molecular techniques. The most resistant isolates (70%) were resist to piperacillin tazobactam and Meropenem, and the less (40%) were resist to Tobramycin. The results also indicated that 30 isolates were MDR and they had ability to produce strong biofilm .
  • Conclusion: The results illustrated that there is a correlation between biofilm formation of organism and antibiotic resistance. Although this a challenge for treatment, it is necessary evaluation of organisms specifically some kinds of opportunistic bacteria like Pseusomonas in the hospitals and find out new medication for remedy of the patients.
  • Keywords: Biofilm, Clinical samples, Pseudomonas