مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Transcriptomic Analysis and Phylogenetic Insights into the Evolution of Venom Allergens in the Hemiscorpius lepturus Scorpion
Transcriptomic Analysis and Phylogenetic Insights into the Evolution of Venom Allergens in the Hemiscorpius lepturus Scorpion
Fatemeh Kazemi-Lomedasht,1,*Mahdi Behdani,2Zohreh Eftekhari,3
1. Venom and Biotherapeutics Molecules Laboratory, Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran 2. Venom and Biotherapeutics Molecules Laboratory, Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran 3. Venom and Biotherapeutics Molecules Laboratory, Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Introduction: Venom allergens have been discerned in various venomous sources such as scorpions, snakes, bees, and wasps, contributing to allergic reactions in humans. This study focused on conducting a phylogenetic analysis of venom allergens extracted from the transcriptome of the Hemiscorpius lepturus scorpion. Seven distinct venom allergens, namely HLAllergen1, HLAllergen2, HLAllergen3, HLAllergen4, HLAllergen5, HLAllergen6, and HLAllergen7, were successfully identified through comprehensive venom gland transcriptome analysis.
Methods: To further elucidate their properties, primary, secondary, and tertiary structures of the identified venom allergens were predicted utilizing advanced computational tools, including ExPASy ProtParam, PSIPRED, and SWISS MODEL servers. The molecular weight of the venom allergens was determined to range between 46 to 52 kDa, and tertiary structure predictions indicated that all 3-D structures fell within the normal range.
Results: A phylogenetic tree, constructed employing MEGA 11 software through the neighbor-joining method with 1000 bootstraps, revealed distinct clades among the identified venom allergens. Specifically, HLAllergen 3, 4, and 5 formed a single clade, while HLAllergen 1, 2, 7, and 6 clustered into separate clades.
Conclusion: Taken together it is imperative to underscore the necessity for further investigations, particularly through proteomic analysis of H. lepturus, to validate and compare the transcriptome-derived data, ensuring a comprehensive understanding of the venom allergen profile in this scorpion species.