مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Investigation of THAP7 and GTF3A genes in glioblastoma cancer in U87 cell line
Investigation of THAP7 and GTF3A genes in glioblastoma cancer in U87 cell line
Kiana Salmani,1Hossein Fahimi,2Ghodratollah (Shahriyar) Panahi,3,*
1. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. 2. Assistant Professor Molecular Genetics, Ph.D. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Azad University, Tehran, Iran. 3. Assistant Professor, Dept.of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran,
Introduction: Glioblastoma multiforme is the most common and aggressive primary tumor in the adult brain. This tumor is formed inside the brain tissue and gradually gets bigger and penetrates the surrounding tissues. Despite years of research and numerous clinical trials, survival is still poor. In this study, using integrated bioinformatics analysis, the most important transcription factors were selected in the first step, and then the expression of the genes obtained from the previous step was studied at the cellular level and in the laboratory on glioblastoma cell lines.
Methods: Analysis of the GSE75147 dataset was conducted in the bioinformatics department to identify genes showing differential expression in glioblastoma. The Enrichr database was utilized to examine signaling pathways. The panther database was used to ascertain the characteristics of the genes. The STRING database was used to analyze protein-protein interactions, while the X2K web database was used to analyze transcription factors and kinases. In conclusion, THAP7 and GTF3A genes were chosen for experimental confirmation in the laboratory. Cell lines U87 and HUVEC were grown in the lab. RNA was isolated and converted into cDNA. Following the design and purchase of primers, the Real-Time PCR method was used to analyze the relative gene expression.
Results: The data of laboratory studies revealed that the THAP7 and GTF3A genes were selected according to the results of bioinformatics analysis Relative expression of THAP7 and GTF3A genes in U-87 cell line were significantly decreased compared to HUVEC cell line.
Conclusion: The results of this study determined THAP7 and GTF3A genes in glioblastoma cell lines can be considered as potential tumor suppressors in glioblastoma cancer.