Introduction: Brucellosis can affect any organ system hearing loss in brucellosis may develope following the involvement of centural auditory pathways or avascular neural tissue due to reflex spams cause by endotoxins . the goal of the treatment brucellosis is to shorten the duration of symptoms and to prevent replase at least two drugs should be used for the treatment of brucellosis at hospital .in present study our patients were treated with antibacterial combination which is in accordance with literature because human brucellosis can affected any regimens vary.
Methods: Since 1999 in japan have been reported .although no bacteria were isolated serum anti body detection indicated that 4were cased by melitensis or B.abortus acquired fatigue .The study compared the BACTEC blood culture system (Becton Dickinson Diagnostic Insrument System Spark) with conventional culture methods for recovery and time to detecetion of significant isolated from normally sterile body fluids a total of 412 specimens were included in the study a significant difference was noted between the blood culture system and routine culture systemand routine culture methods for recovery of pathogenic .microorganisms that were from sterilebody fluids the most frequently isolated microorganisms recovery with both culture methods .
Results: Contact with infected animals consumptions of raw milk and ignorance regarding the disease are the major risk factors. since clinical manifestions of this disease are protein in nature Rose bengal platetest(RBPT)and serum aggulatinationtest(SAT ).
Conclusion: Brucella were recovered only with blood culture system further more the mean time to detection of signification pathogens was significantly less with blood culture system than with conventional media BACTEC blood culture system was found to improve the yield of clinically significant isolated from normally sterile body fluids with reduced time to detection it maybe adanatageous for isolation of fastidiuos microorganisms such as brucella and S.pnemoniae especially from cerebrospinal and synovial fluids speciemens.