• Assessing miRNA-217 overexpression effects on type 1, type 2, and type 3 inositol trisphosphate receptor gene expression in B (Raji) and T (Jurkat) acute lymphoblastic leukemia cell lines
  • Mahboobeh Hayati,1 Narges Obeidi,2 Mohammad Javad Mousavi,3 Gholamreza Khamisipour,4,*
    1. Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
    2. Department of Hematology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
    3. Department of Hematology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
    4. Department of Hematology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran


  • Introduction: Acute lymphoblastic leukemia (ALL) is a malignant transformation and proliferation of lymphoid progenitor cells in the bone marrow, blood, and extramedullary sites. MicroRNAs, which are involved in the regulation of cellular processes such as proliferation, invasion, metastasis, and apoptosis, have been implicated in cancer development. MiRNA-217, in particular, has been identified as a potential anticancer factor owing to its decreased expression levels in several cancers. The inositol 1,4,5-trisphosphate receptors (IP3Rs), which are involved in the IP3/calcium signaling pathway, play important roles in the regulation of cellular functions such as proliferation, apoptosis, differentiation, and metabolism. Moreover, they have been linked to neoplastic transformation and progression. Therefore, this study aimed to investigate the effect of miR-217 overexpression on the gene expression of IP3Rs in Jurkat and Raji cell lines.
  • Methods: Jurkat and Raji Cell lines were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and incubated under controlled conditions of 37 °C, 95% humidity, and 5% carbon dioxide. In addition, the normal fibroblast cell line was cultured in DMEM. Next, miR-217 was transduced into the cells using lentiviral vectors. RNA was extracted from the cells 48 and 72 h after transduction, and complementary DNA (cDNA) was synthesized. Finally, transduction efficiency was confirmed using real-time qPCR, and the mRNA levels of IP3Rs genes were measured using real-time qPCR.
  • Results: The expression levels of IP3R1 and IP3R3 genes were found to be decreased in the miR-217 transduced group compared to the control group in all three cell lines at 48 and 72 hours after transduction. This decrease was significant in all three cell lines at 48 h, except for IP3R3 expression in Raji cells, which was only significantly decreased at 72 h after transduction. Furthermore, the expression of the IP3R2 gene in the miR-217 transduced group compared to the control group increased at 48 h but decreased at 72 h in Jurkat cells, whereas it increased in Raji cells and decreased in fibroblast cells at both 48 and 72 h after transduction.
  • Conclusion: The results of the current study demonstrated that the expression of IP3R1, IP3R2, and IP3R3 mRNA is altered with the overexpression of miR-217 in Jurkat, Raji, and Fibroblast cells, suggesting a potential role of miR-217 in regulating the expression of these genes. This is important for understanding the molecular mechanisms that form the basis of various cellular processes. Further studies are needed to understand the role of miR-217 in the IP3/calcium signaling pathway.
  • Keywords: Acute lymphoblastic leukemia, hsa-mir-217, Inositol 1,4,5-Trisphosphate Receptors