مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Application of the CRISPR/Cas9 System for repair or regeneration articular cartilage
Application of the CRISPR/Cas9 System for repair or regeneration articular cartilage
sadaf safaei,1,*Hamid Mir Mohammad Sadeghi,2
1. Department of pharmaceutical biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of pharmaceutical biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: Cartilage is a specialized form of connective tissue. The three types of cartilage is hyaline (most common type of cartilage), elastic, and fibrocartilage. A thin layer of hyaline cartilage covers articulating surface of each bone, with a dense extracellular matrix and scattered chondrocytes forms the articular cartilage (AC). Articular cartilage has a limited capacity for self-renew and repair, therefore, find a potential treatment for cartilage damage is a challenge. Current methods used for cartilage damage and repair include a range of rest, medications, surgery. These methods couldn’t defects for long-term to achieve therapeutic effects for a long time. In this review, we discuss the CRISPR/Cas9-based system to enhance long-term therapeutic effects.
Methods: Current methods used for cartilage damage and repair include a range of rest, medications, surgery. CRISPR/Cas9 system as the most flexible and user-friendly platform to generate genome editing technology, overcome the current limitations. CRISPR/Cas9 system generate target gene modifications: insertion, knockout (deletion) and genome editing.
Results: CRISPR/Cas9 technology has been used to generate modifications on target genome can overcome many limitations of traditional strategies. It can provide the ability to regulate genome sequence. There are two main strategies insert to generate modifications on target genome by CRISPR/Cas9 system: First, modified genome in vitro on osteoblasts or chondroblasts, modified genome loaded in the vector and applied directly to the site. Second, use ex vivo methods: generate modifications on suitable tissues and then re implanted in vivo. Genome editing based on CRISPR/Cas9- system has provided similar natural mutation and low off target effects.
Conclusion: At present, CRISPR/Cas9 technology in articular cartilage has the ability to establish deletion (Knock Out) or insertion (Knock In) of specific genomic sequences on a single step directly applied in defects which makes it safe and effective. CRISPR/Cas9 system can blockade of certain cytokines, generate similar natural mutation and low off target effects and overcome many limitations of traditional strategies.