• Investigation of acute toxicity and abnormality of reproductive system by cisplatin in rat
  • Amirhossein Yazdi,1 Mohammadhossein eshaghi ghalibaf,2,* Afsaneh hokmabadi,3
    1. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
    2. Phd student of medical physiology.department of medical physiology -mashhad university of medical science .iran.
    3. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.


  • Introduction: Infertility is defined as the inability to achieve pregnancy after one year of unprotected sex. Male infertility is the main cause of this issue and various factors such as hormonal imbalance, genetic factors and environmental factors play a role in it. Exposure to a variety of drugs, including chemotherapy drugs, has been linked to male fertility problems, including infertility and reproductive disorders. Cisplatin is a very effective chemotherapy drug used to treat most solid tumors. But one of its side effects is testicular poisoning, which can lead to fertility abnormalities. This study examines the effects of cisplatin toxicity in Wistar rat infertility and highlights the potential risks associated with exposure to chemotherapy drugs and the need for greater attention to drugs in reproductive health.
  • Methods: In this study, 36 adult male Wistar rats weighing about 200to 350grams were obtained from the animal house of Mashhad University of Medical Sciences. The animals had free access to food and water during the study. Then the animals were randomly assigned to 3 groups of 12 were divided and were dosed for 14 days. They were randomly divided into three groups of 12 each. The first group of the control group received normal saline intraperitoneally with a dose of 4 mg/kg, the second group received cisplatin with a dose of 4 mg/kg intraperitoneally. was injected and the third group of cisplatin group was injected intraperitoneally with a dose of 7mg/kg.After 14 days, rats were anesthetized and sacrificed with carbon dioxide for weighing and tissue sampling, and after that, testis, seminal vesicle, epididymis, vas deferens and prostate tissues were separated.
  • Results: After 14 days of cisplatin injection with a dose of 4mg/kg and a dose of 7 mg/kg, which were under dose monitoring, the dose of 4 mg/kg did not cause abnormalities, but we came to the conclusion that the injection of a dose of 7 mg/kg caused necrosis and Damage to the seminiferous tubules also caused a decrease in the index of the testis, seminal vesicle, epididymis, vas deferens and prostate.
  • Conclusion: Studies have shown that chemotherapy drugs such as cisplatin can cause atrophy of the reproductive organs as well as abnormality of testicular germ cells including spermatogonial cells and spermatids, and by producing free radicals, oxidative stress and DNA damage, it causes abnormality of the reproductive system.
  • Keywords: reproductive system_rat _Cisplatin