مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
The genotyping of Human Papilloma Virus (HPV) in Women's Cervical Tissue Samples from Fasa, Iran
The genotyping of Human Papilloma Virus (HPV) in Women's Cervical Tissue Samples from Fasa, Iran
Mozhgan Ahmadzadeh,1Abdolreza Mohebbi,2,*
1. Department of cellular and molecular biology, faculty of biological sciences, Kharazmi university , Tehran Iran. 2. Department of Medical, Faculty member of Fasa University of Medical Siences, Fars, Iran.
Introduction: Human papillomavirus (HPV) is the most prevalent sexually transmitted infection globally, with more than 100 different types identified so far. According to the (World Health Organization) WHO reports Cervical cancer is the fourth most common cancer in women globally.
Methods: In order to conduct this study, tissue samples were obtained from 756 patients who had 2017 to 2022 referred for HPV screening by gynecologist.In this study, after collecting the samples, DNA was extracted from the samples, then genotyping of the investigated samples was done using the Real Time PCR method.The detection rate of the HPV genome in women's cervical tissue samples was evaluated using Graph Pad/Prism8.0.2 software and documented the findings in a written report.
Results: This study analyzed the demographic and HPV genotyping data of 755 women cases. The majority of cases were in the age groups of 31-40 years and 21-30 years. HPV testing by Real-Time PCR showed a positivity rate of 53.11%, with the highest prevalence in the 31-40 years’ age group followed by the 21-30 years’ age group.
Cytology analysis revealed that LSIL was the most common lesion, followed by HSIL and ASCUS among HPV-positive samples. HPV genotyping identified HPV-6 and HPV-16 as the most common monotypes, while HPV-6,11 predominated in mix genotypes cases. The combination of cytology and HPV genotyping results showed distinct patterns of HPV types in LSIL, ASCUS, and HSIL samples.
Conclusion: The results of this study contribute valuable insights into the epidemiology of HPV infection and its association with cytological abnormalities in cervical lesions.