مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Decellularized sheep peritoneum: a good scaffold for in vitro cell culture
Decellularized sheep peritoneum: a good scaffold for in vitro cell culture
Zeinab Shafiei Seifabadi,1,*Dian Dayer,2Vahid Bayati,3Nilufar Lotfian,4Abbass heidari-moghadam5,5
1. Behbahan Faculty of Medical Sciences, Behbahan, Iran. 2. Cellular and molecular research center, Medical Basic Sciences Research Institute, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 3. Cellular and molecular research center, Medical Basic Sciences Research Institute, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 4. Cellular and molecular research center, Medical Basic Sciences Research Institute, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 5. Department of Anatomical Sciences, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
Introduction: The search for optimal biomaterials for tissue engineering and cell culture has led to the exploration of various natural scaffolds. This study investigates the potential of decellularized sheep peritoneum as a scaffold for in vitro cell culture. Decellularization, a process that removes cellular components while preserving the extracellular matrix, was applied to sheep peritoneum to create a biocompatible and non-immunogenic scaffold.
Methods: The decellularized tissue was characterized using histological, biochemical, and mechanical analyses to assess its structural integrity and cellular compatibility. Subsequent in vitro cell culture experiments were conducted to evaluate the scaffold's ability to support cell attachment, proliferation, and differentiation.
Results: The histological examination of hematoxylin and eosin and Masson's trichrome stained samples of decellularized and native peritoneum reveals complete decellularization while maintaining the extracellular matrix microarchitecture after 4 days. Additionally, DAPI staining confirms the complete removal of DNA fragments from the tissue samples. Scanning electron microscopy shows that the 3D ultrastructure of the peritoneum, characterized by a porous appearance, is well-preserved following decellularization. Microscopic analysis of in vitro cultured scaffolds indicates the viability of adipose-derived stem cells (ADSC).
Conclusion: The results indicate that decellularized sheep peritoneum provides a favorable microenvironment for cell growth, demonstrating its potential as a viable scaffold for tissue engineering applications. This study highlights the feasibility of using decellularized sheep peritoneum in biomedical research and its potential for future clinical use in regenerative medicine.