مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Inhibitory Effects Of Marine Metabolites Against Some Uropathogenes and Beta-Lactamases
Inhibitory Effects Of Marine Metabolites Against Some Uropathogenes and Beta-Lactamases
Hoda Khaledi,1,*Noura Oude Obeid,2
1. Iranian National Institute for Oceanography and Atmospheric Science, Tehran, Iran 2. Master Student of Microbiology, Department of Biology, College of Convergent Sciences and Technologies, Islamic Azad University, Science and Research Branch
Introduction: Urinary tract infection is a common disease often caused by Escherichia coli bacteria. This bacterium produces beta-lactamases that resist antibiotics. Marine actinomists can produce a wide range of metabolites, which can be used as antibiotics.
Methods: E. coli phylogenetic grouping was done using PCR on three markers yjaA, chuA, and TspE4.C2. 300 Samples of patients were collected and identified by testing the diagnostic tests of E. coli. Anti biogram test was measured by using common antibiotics, and extracts of marine actinomist bacterial extract, on bacteria. 50 isolates were identified and tested for sensitivity using antimicrobial agents CLSI, 2021.
Results: 32/50 (64 percent) of the isolates were resistant to several drugs and 18/50 (36 percent) were non-resistant. The inhibitory effect of actinomycet extract against 10 resistant E. coli isolates was observed. Prevalence (gene blaOXA 46%), (gene blaTEM 68%), (gene blaCTXM-IV 68%), (gene blaNDM 26%), (gene blaCTXM-II 50%), (gene blaCTXM-I 100%) was reported in multi-antibiotic resistant samples. The phylogenetic grouping showed that E. coli's isolates consisted of group B2 (84%), subgroup B1 (6%), group A (2%), group D (8%), and 72%-100% of the isolates were resistant to cephalosporin.
Conclusion: This resistance indicates the pressure of possible antibiotic selection in the community. Pathogen resistance factors to beta-lactam were often in the phylogenetic group B2, and this group had the highest number of blaCTX-M and blaTEM genes in resistant isolates. Marine Actinomistasts showed great ability in inhibiting resistant isolates, so they could be used as a suitable alternative to the treatment of resistant E. coli.