مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
using viruses in CRISPR
using viruses in CRISPR
Atefeh Bozorg Panah,1,*
1. Bachelor’s student, Microbiology group, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Introduction: Introduction
The CRISPR-Cas systems have fundamentally transformed the field of genetic engineering by offering precise methodologies for genome modification. Initially identified as an immune response mechanism in bacteria, CRISPR-Cas has been repurposed for application across diverse biological organisms. Viruses serve an essential function in augmenting the efficiency and specificity of CRISPR-Cas implementations. This article examines the incorporation of viruses within CRISPR-Cas frameworks, emphasizing their methodologies, outcomes, and prospective implications for future research.
Methods: Methods
The incorporation of viruses in CRISPR-Cas entails the utilization of viral vectors to transport CRISPR constituents into designated target cells. These vectors, including lentiviruses and adenoviruses, are meticulously engineered to encompass the requisite CRISPR components, such as the Cas protein and guide RNA. The viral-mediated delivery approach provides numerous advantages, including elevated transduction efficiency and the capacity to target a wide array of cell types. The formulation of these viral vectors necessitates rigorous design protocols to ensure safety and to mitigate off-target effects.
Results: Results
The amalgamation of viral vectors with CRISPR-Cas systems has culminated in substantial advancements within the realms of biomedical research and therapeutic interventions. The utilization of viral delivery mechanisms has augmented the accuracy of gene editing techniques in both in vitro and in vivo experimental frameworks. This methodology has been employed to rectify genetic aberrations, elucidate gene functionality, and establish disease models. The findings reveal an increase in editing efficacy alongside a diminished immunological response in contrast to conventional methodologies. Furthermore, the incorporation of viral vectors has broadened the prospective applications of CRISPR-Cas technology in the management of genetic disorders and infectious pathologies.
Conclusion: Conclusion
The integration of viral entities with CRISPR-Cas systems signifies a groundbreaking domain within the field of genetic engineering. By utilizing the inherent mechanisms of viral infection, scholars can attain a more efficient and precise delivery of CRISPR components. This collaboration possesses the capacity to enhance therapeutic interventions and enrich our comprehension of gene functionalities. Nevertheless, continuous inquiry is imperative to confront challenges including safety issues and ethical dilemmas. As technological advancements unfold, the involvement of viruses in CRISPR-Cas applications is anticipated to broaden, thereby facilitating novel approaches to intricate genetic issues.