مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Functional dopaminergic neurons derived from humanchorionic mesenchymal stem cells ameliorate striatalatrophy and improve behavioral deficits in Parkinsonianrat model
Functional dopaminergic neurons derived from humanchorionic mesenchymal stem cells ameliorate striatalatrophy and improve behavioral deficits in Parkinsonianrat model
Introduction: Human chorionic mesenchymal stem cells (HCMSCs) have been recognized as a desirable choice for cell therapy in neurological disorders such as Parkinson's disease (PD). Due to invaluable features of HCMSCs including their immunomodulatory and immunosuppressive properties, easily accessible and less differentiated compared to other types of MSCs, HCMSCs provide a great hope for regenerative medicine.
Methods: Initially, HCMSCs were isolated and underwent a 2-week DA differentiation, followed by in vitro assessments, using quantitative real-time polymerase chain reaction, immunocytochemistry, patch clamp recording, and high-performance liquid chromatography. In addition, the effects of implanted HCMSCs-derived DA neuron-like cells on the motor coordination along with stereological alterations in the striatum of rat models of PD were investigated.
Results: The results showed that under neuronal induction, HCMSCs revealed neuron-like morphology, and expressed neuronal and DA-specific genes, together with DA release. Furthermore, transplantation of HCMSCs-derived DA neurons into the striatum of rat models of PD, augmented performance. Besides, it prevented reduction of striatal volume, dendritic length, and the total number of neurons, coupled with a diminished level of cleaved caspase-3.
Conclusion: Altogether, these findings suggest that HCMSCs could be considered as an attractive strategy for cell-based therapies in PD.