مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Examining the amount of expression Circ-FOXM1 and miR-149-5p in patients with ovarian cancer
Examining the amount of expression Circ-FOXM1 and miR-149-5p in patients with ovarian cancer
Shaghyegh hoseini,1,*Maryam rahmani,2
1. Department of Biology,North Tehran Branch, Islamic Azad University, Tehran, Iran 2. Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
Introduction: Ovarian cancer is one of the most feared malignancies in women. The use of new biomarkers for faster diagnosis and better treatment selection is one of the main concerns in this field, so it shows the necessity of new diagnostic and treatment methods.
Methods: Preparation of cancer tissue and in this study 50 fresh tissue samples consisting of 25 cancer samples and 25 healthy marginal tissue samples from cancer patients in different stages of the disease are used to investigate the expression of miR-149-5p and circ-FOXM1. RNA extraction from the cell is done manually. We use the nano drop device to check the quality and also to determine the concentration. To investigate the expression changes of miR-149-5p and circ-FOXM1, first, circ-FOXM1 synthesis is performed using the kit. Primer-BLAST software is also used to check primers. The next step is real-time reaction or qRT PCR using Master Mix Cyber Green and finally the results are analyzed.
Results: One way ANOVA is used to compare each of the obtained variables. In this study, the p value is less than 0.05, it is considered statistically significant. Also, adjustment of the test is done at the same time using the Bonferroni method. In addition, the correlation between two gene expression rates is done using Pearson's correlation coefficient.
Conclusion: Due to the fact that the expression and interpretation of 2 biomarkers miR-149-5p and circ-FOXM1 have not been investigated simultaneously in ovarian cancer, as a result, this study is new in its turn.