مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
The Protective Efficacy of Curcuma longa and Pioglitazone in Alleviating Paraquat-Induced Lung Injury in Rats
The Protective Efficacy of Curcuma longa and Pioglitazone in Alleviating Paraquat-Induced Lung Injury in Rats
Mohammad Hossein eshaghi ghalibaf,1,*Amirhossein yazdi,2Mohammad Hossein boskabady,3Seyedeh-Najibeh Nasiri,4
1. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 3. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 4. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Introduction: Inhalation of paraquat (PQ) is one of the most widely used Herbicides in the world can cause lung damage. In recent years, extensive in studies suggested curcumin has anticancer, antiviral, antiarthritic, anti-amyloid, antioxidant, and anti-inflammatory properties Curcuma longa (Cl) has a long history in traditional and folk medicine for the treatment of a wide range of disorders, including respiratory diseases.The aim of this study was to investigate the preventive effect of curcumin on lung damage caused by inhaled PQ in rats.
Methods: Male Wistar rats were divided into 8 groups (n = 5), one group exposed to saline (control) and other groups exposed to PQ aerosol. Saline (PQ), Cl extract, (two doses), curcumin (Cu), pioglitazone (Pio), and the combination of Cl‐L + Pio and dexamethasone (Dex) were administered during the exposure period to PQ. Total and differential white blood cell (WBC) counts, oxidant and antioxidant indicators in the bronchoalveolar lavage (BALF), and tumor necrosis alpha (TNF‐α) levels in the lung histologic , and air way responsiveness to methacholine were evaluated.
Results: WBC counts (Total and differential), malondialdehyde level, tracheal responsiveness (TR), TNF‐α and histopathological changes of the lung were markedly elevated but total thiol content and the activities of catalase and superoxide dismutase were decreased in the BALF in the PQ group. Both doses of Cl, Cu, Pio, Cl‐L + Pio, and Dex markedly improved all measured variables in comparison with the PQ group.
Conclusion: CI, Pio, and Cl‐L + Pio improved PQ‐induced lung inflammation and oxidative damage.Histopathological assessments revealed that the combination of Cl-L + Pio significantly mitigated lung tissue damage caused by paraquat exposure. Additionally, the treatments resulted in improved airway responsiveness to methacholine, suggesting enhanced pulmonary function.