مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Optical Microscopy Imaging: Unveiling the Molecular Landscape of Biological Systems
Optical Microscopy Imaging: Unveiling the Molecular Landscape of Biological Systems
Mohammadreza Elhaie,1,*Abolfazl Koozari,2Iraj Abedi,3
1. Department of Medical Physics, School of Medicine Isfahan University of Medical Sciences 2. Department of Medical Physics, School of Medicine Ahvaz Jundishapur University of Medical Sciences 3. Department of Medical Physics, School of Medicine Isfahan University of Medical Sciences
Introduction: Optical microscopy imaging has emerged as a powerful tool for visualizing and tracking individual molecules or nanoparticles within biological environments. This technique offers high temporal and spatial resolution, enabling researchers to study biological processes and detect biomolecules with unprecedented detail. The application of optical microscopy imaging has revolutionized various fields, including molecular detection and single-particle tracking.
Methods: A comprehensive literature search was conducted using various databases, including PubMed, Semantic Scholar, arXiv, and NCBI PMC. The search terms included "optical microscopy imaging," "super-resolution microscopy," "single-molecule tracking," "photothermal microscopy," "nonlinear optical microscopy," "photoacoustic microscopy," and "supercontinuum radiation." Relevant articles published in peer-reviewed journals were carefully reviewed and analyzed.
Results: Several optical microscopy techniques have been developed and employed in bio-analytical assays. Total internal reflection fluorescence microscopy (TIRFM), super-resolution optical microscopy (SRM), and dark-field optical microscopy (DFM) are among the widely used techniques. These methods utilize principles such as fluorescence, absorption, and scattering to generate high-resolution images of biological samples. Photothermal microscopy has also been explored for label-free detection of individual nanoabsorbers, overcoming limitations of fluorescence-based detection. Other techniques like nonlinear optical microscopy, photoacoustic microscopy, and supercontinuum radiation have further expanded the capabilities of optical microscopy imaging.Optical microscopy imaging has been successfully applied in various fields, enabling the study of biological processes and the detection of biomolecules with high spatial and temporal resolution. These techniques offer several advantages, including non-invasive and non-destructive imaging of biological samples, real-time observation of live cells and tissues, and label-free detection capabilities. However, challenges such as the need for high-quality optics, precise control over imaging conditions, and the potential for photobleaching or photodamage to the sample need to be addressed.
Conclusion: Optical microscopy imaging has become an indispensable tool in bio-analytical assays, providing researchers with a powerful means to study biological systems and improve diagnostic capabilities. The development of new techniques and methods, coupled with ongoing efforts to address existing challenges, will further enhance the sensitivity, resolution, and applicability of optical microscopy imaging in various fields.