مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
The Effect of Remdesivir on STAT3 gene expression in patients with covid-19
The Effect of Remdesivir on STAT3 gene expression in patients with covid-19
Sepideh Mahdavi,1Somayeh Arabzadeh,2,*Sohameh Mohebbi,3
1. Department of Biology, Faculty of Basic Science, Ale Taha Institute of Higher Education, Tehran, Iran 2. Department of Biology, Faculty of Basic Science, Ale Taha Institute of Higher Education, Tehran, Iran 3. Department of Biology, Faculty of Basic Science, Ale Taha Institute of Higher Education, Tehran, Iran
Introduction: Abstract
In 2019, Covid-19 spread rapidly and was recognized as a pandemic. In the conducted studies, it was observed that the activation of inflammatory pathways in patients is closely related to the severity of the disease; Therefore, investigating the inflammatory pathway caused by this virus is necessary for treatment against COVID-19. One of the main inflammatory pathways in COVID-19 is the jak/stat signaling pathway. Remdesivir is one of the antiviral drugs that has been widely investigated for the treatment of this disease. This research aims to examine the changes in STAT3 gene expression as one of the key factors in activated inflammatory pathways in patients with COVID-19 before and after treatment with remdesivir compared to healthy individuals. In this study, blood samples were randomly taken from 30 patients infected with the Omicron strain of coronavirus, before receiving the remdesivir drug as a patient group and after receiving the last dose of drug as a treatment group and from 20 healthy people as a control group. After receiving the samples, RNA extraction and cDNA synthesis were performed. Changes in STAT3 gene expression were investigated by real-time PCR method. The level of STAT3 gene expression decreased by 80% in the patient group and by 20% in the group treated with remdesivir compared to the control group (P<0.001). The findings of this research indicate the positive effect of the remdesivir drug in increasing the expression of the STAT3 gene in patients with COVID-19, while it could not increase the expression of this gene to the level of the control group.
Methods: Abstract
In 2019, Covid-19 spread rapidly and was recognized as a pandemic. In the conducted studies, it was observed that the activation of inflammatory pathways in patients is closely related to the severity of the disease; Therefore, investigating the inflammatory pathway caused by this virus is necessary for treatment against COVID-19. One of the main inflammatory pathways in COVID-19 is the jak/stat signaling pathway. Remdesivir is one of the antiviral drugs that has been widely investigated for the treatment of this disease. This research aims to examine the changes in STAT3 gene expression as one of the key factors in activated inflammatory pathways in patients with COVID-19 before and after treatment with remdesivir compared to healthy individuals. In this study, blood samples were randomly taken from 30 patients infected with the Omicron strain of coronavirus, before receiving the remdesivir drug as a patient group and after receiving the last dose of drug as a treatment group and from 20 healthy people as a control group. After receiving the samples, RNA extraction and cDNA synthesis were performed. Changes in STAT3 gene expression were investigated by real-time PCR method. The level of STAT3 gene expression decreased by 80% in the patient group and by 20% in the group treated with remdesivir compared to the control group (P<0.001). The findings of this research indicate the positive effect of the remdesivir drug in increasing the expression of the STAT3 gene in patients with COVID-19, while it could not increase the expression of this gene to the level of the control group.
Results: Abstract
In 2019, Covid-19 spread rapidly and was recognized as a pandemic. In the conducted studies, it was observed that the activation of inflammatory pathways in patients is closely related to the severity of the disease; Therefore, investigating the inflammatory pathway caused by this virus is necessary for treatment against COVID-19. One of the main inflammatory pathways in COVID-19 is the jak/stat signaling pathway. Remdesivir is one of the antiviral drugs that has been widely investigated for the treatment of this disease. This research aims to examine the changes in STAT3 gene expression as one of the key factors in activated inflammatory pathways in patients with COVID-19 before and after treatment with remdesivir compared to healthy individuals. In this study, blood samples were randomly taken from 30 patients infected with the Omicron strain of coronavirus, before receiving the remdesivir drug as a patient group and after receiving the last dose of drug as a treatment group and from 20 healthy people as a control group. After receiving the samples, RNA extraction and cDNA synthesis were performed. Changes in STAT3 gene expression were investigated by real-time PCR method. The level of STAT3 gene expression decreased by 80% in the patient group and by 20% in the group treated with remdesivir compared to the control group (P<0.001). The findings of this research indicate the positive effect of the remdesivir drug in increasing the expression of the STAT3 gene in patients with COVID-19, while it could not increase the expression of this gene to the level of the control group.
Conclusion: Abstract
In 2019, Covid-19 spread rapidly and was recognized as a pandemic. In the conducted studies, it was observed that the activation of inflammatory pathways in patients is closely related to the severity of the disease; Therefore, investigating the inflammatory pathway caused by this virus is necessary for treatment against COVID-19. One of the main inflammatory pathways in COVID-19 is the jak/stat signaling pathway. Remdesivir is one of the antiviral drugs that has been widely investigated for the treatment of this disease. This research aims to examine the changes in STAT3 gene expression as one of the key factors in activated inflammatory pathways in patients with COVID-19 before and after treatment with remdesivir compared to healthy individuals. In this study, blood samples were randomly taken from 30 patients infected with the Omicron strain of coronavirus, before receiving the remdesivir drug as a patient group and after receiving the last dose of drug as a treatment group and from 20 healthy people as a control group. After receiving the samples, RNA extraction and cDNA synthesis were performed. Changes in STAT3 gene expression were investigated by real-time PCR method. The level of STAT3 gene expression decreased by 80% in the patient group and by 20% in the group treated with remdesivir compared to the control group (P<0.001). The findings of this research indicate the positive effect of the remdesivir drug in increasing the expression of the STAT3 gene in patients with COVID-19, while it could not increase the expression of this gene to the level of the control group.