مقالات پذیرفته شده در هشتمین کنگره بین المللی زیست پزشکی
Unraveling the Impact of Long Non-Coding RNAs on Protein Networks in Colorectal Cancer: A New Frontier in Cellular Dynamics
Unraveling the Impact of Long Non-Coding RNAs on Protein Networks in Colorectal Cancer: A New Frontier in Cellular Dynamics
Elham Shaarbaf Eidgahi,1Alireza Pasdar,2Pouria Abidi,3Forouzan Amerizadeh,4,*
1. 1. Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2. 2. Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 3. 3. Student Committee of Medical Education Development, Education Development Center, Mashhad University of Medical Sciences, Mashhad, Iran 4. 5. Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Introduction: LncRNAs play a pivotal role in colorectal cancer by serving as key regulators of gene expression, with their dysregulation linked to various cancer hallmarks. This study focuses on unraveling the molecular underpinnings of colorectal cancer susceptibility by deciphering dysregulated LncRNAs that target the PI3K/AKT pathway.
Methods: We conducted an extensive review of prior studies exploring the modulatory role of dysregulated LncRNAs in CRC. Utilizing the LncRNome database, we identified all LncRNA-targeted genes. Cytoscape software facilitated the investigation of protein-protein interactions (PPI) among these genes, with a focus on identifying hub proteins. Gene-subnetwork Gene Ontology (GO) analysis, Cytocluster analysis, and promoter motif analysis were performed for a comprehensive understanding.
Results: Our analysis revealed genes targeted by dysregulated LncRNAs impacting the Wnt pathway. Within this network, 12 hub proteins were identified. Subnetwork analysis showcased five crucial functional modules, complemented by the identification of 10 significant promoter motif elements through Cytocluster analysis and promoter motif analysis.
Conclusion: This study not only contributes to understanding the intricate regulatory mechanisms of dysregulated LncRNAs in colorectal cancer but also paves the way for identifying potential biomarkers and therapeutic targets. The findings hold promise for advancing personalized approaches in the management of colorectal cancer.