• Investigation of LINC01484 expression in gastric cancer
  • Zohreh Al-Zahra Abdellahi,1 Tahereh Sadeghian-Rizi,2,* Mansoureh Azadeh,3
    1. Department of Genetics, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran
    2. Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran
    3. دپارتمان زیست فناوری نوین-اصفهان


  • Introduction: Gastric cancer is the one most common cancer in the world. In most cases, the disease is diagnosed at a stage when common therapies do not have a significant impact on the patient's life. Therefore, the need to study effective molecular mechanisms to introduce molecular markers for rapid detection of prognosis is strongly felt. Long intergenic noncoding RNAs (lincRNAs) are of paramount importance in the underlying molecular mechanisms of cancer initiation and progression. They can be used as molecular biomarkers to diagnose and track disease progression or as therapeutic targets. The oncogenic role of LINC01484 in various cancers such as bladder urothelial carcinoma has been identified. According to the bioinformatics studies, LINC01484 may be involved in the gastric progression and metastasis. For this reason, the aim of this study was investigation of LINC01484 expression in stomach tissue samples and evaluation of its potential as diagnostic biomarker in this cancer for first time.
  • Methods: The gastric tumor samples and adjacent normal tissue samples were collected from thirty patients. After total RNA extraction from samples and cDNA synthesis, Real-time PCR was used to measure the LINC01484 expression. Paired t-test was used for comparison of LINC01484 expression in tumor and normal samples and receiver operating curve (ROC) was used to assess the diagnostic value of LINC01484.
  • Results: The qPCR results showed that the expression of LINC01484 was up-regulated in thirty paired gastric cancer specimens (LogFC=2.399, p-value = 0.0168). The AUC of ROC curve was 0.7 (p-value=0.016) and revealed that the expression level of LINC01484 can detect up to 70% of cases of gastric cancer and can be used as a diagnostic biomarker.
  • Conclusion: Finally, the authors suggest that the considerably up-regulation of LINC01484 in gastric tumor samples and its potential as biomarker highlight the need to further investigation of this lncRNA in larger population and cohort studies.
  • Keywords: LINC01484, Gastric cancer, cancer progression, cancer metastasis, qPCR