• Differential expression of steroidogenesis-related gene CYP19A1 in adipose tissue adjacent to benign and malignant breast tumors
  • Mahdis Danaei,1,* Mona Mirzaei,2 Raha Favaedi,3 Alireza Alizadeh,4 Sadaf Alipour,5 Maryam Shahhoseini,6
    1. Department of Molecular Genetics, Faculty of Basic Sciences and advanced Technologies in Biology, University of Science and Culture, Tehran, Iran
    2. Department of Molecular Genetics, Faculty of Basic Sciences and advanced Technologies in Biology, University of Science and Culture, Tehran, Iran
    3. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
    4. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
    5. Breast Diseases Research Center (BDRC), Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran -Department of Surgery, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran
    6. Department of Molecular Genetics, Faculty of Basic Sciences and advanced Technologies in Biology, University of Science and Culture, Tehran, Iran Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran


  • Introduction: Breast cancer is the most commonly diagnosed malignant tumor in women and the second leading cause of cancer-related deaths worldwide. The breast is rich of adipose tissue and glandular tissue. Because adipocyte and tumor cells are so closely located in the breast, it's important to explore how adipocyte may influence the onset, growth, and spread of breast cancer at the molecular level. A major aspect of focusing throughout this study is the genes express adipose tissue that regulate steroid hormones, such as those involved in producing mineralocorticoids, glucocorticoids, and sex steroids. Our specific target is the CYP19A1 gene, which plays a critical role in the metabolism of sex steroids, especially in producing estrogen. An evaluation of expression the CYP19A1 gene in the adipose tissue surrounding breast tumors.
  • Methods: We collected samples of subcutaneous adipose tissue adjacent to breast tumors from 40 women with breast cancer. We divided them two،two groups had a history of benign (non-cancerous) tumors, and the other half had malignant (cancerous) breast tumors. are main goal was to compare CYP19A1 gene expression between in adipose tissue surrounding benign and malignant breast tumors. by quantitative PCR technique, a method for analyzing gene expression.
  • Results: Our results indicated that CYP19A1 profile expression, was significantly higher in patients with malignant breast tumors compared to those with benign breast cancer. There was no significant different in BMI and age between 2 groups ( benign tumors and malignant tumors).
  • Conclusion: The study revealed that alterations in the expression of the CYP19A1 gene are linked to an increased risk of developing more aggressive breast cancer. Upregulation of CYP19A1 genes increased boosting malignancy causing breast tumor.
  • Keywords: Breast cancer, fat tissue, gene expression, aromatase, CYP19A1.