Investigating the effect of doxycycline antibiotic on the process of angiogenesis of the chorioallantoic membrane of chick embryos
Investigating the effect of doxycycline antibiotic on the process of angiogenesis of the chorioallantoic membrane of chick embryos
Mohammad Reza Pour Mohammad,1,*Jina Khayat Zadeh,2Hannaneh Akbari,3Sima Afsharnezhad,4Parsa Farrokhi,5
1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran 2. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran 3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran 4. Department of biochemistry , Mashhad Branch, Islamic Azad University, Mashhad, Iran 5. Faculty of medicine, Ilam University of Medical Sciences, Ilam, Iran
Introduction: Angiogenesis refers to the biological process of sprouting new vessels from existing vessels in the tissue. Angiogenesis is a physiological process that is highly regulated and occurs in cases such as wound healing, menstrual cycles, placental growth, and ovulation Doxycycline antibiotic has a wide biomedical application compared to other antibiotics. This research was conducted with the aim of investigating the effect of doxycycline antibiotic on the process of angiogenesis of the chorioallantoic membrane of chick embryos.
Methods: In this research, 40 Ross spray eggs were randomly divided into 5 groups including control, laboratory control (pbs) and 3 experimental groups. After incubation, on the second day of the window, an egg was created and in on the eighth day, after placing the gelatin sponge on the chorioalantoic curve, doxycycline antibiotic was injected with doses (50, 100 and 250 micromole/ milliliter) on the chorioallantoic membrane of chick embryos. On the twelfth day, the corioalantoic membrane was taken and length, the number of vascular splits, weight and height of the embryos were measured. The collected data were analyzed by Excel and SPSS 20 statistical software.
Results: The average number and total length of vascular branches in the laboratory control group did not show any significant difference compared to the control group. The average number and length of vascular branches in concentrations of 50, 100 and 250 micromole/ milliliter of doxycycline antibiotic showed a significant decrease compared to the control group.
Conclusion: According to this study, the doxycycline antibiotic has an inhibitory effect on angiogenesis in the chorioallantoic membrane of chick embryos. Also, it seems that the antibiotic doxycycline can be used to inhibit angiogenesis in cancer tissues.