• Investigating the effect of doxycycline antibiotic on the expression of FGF and VEGF genes in the chorioallantoic membrane of chick embryos by Real time PCR
  • Mohammad Reza Pour Mohammad,1,* Jina Khayat Zadeh,2 Hannaneh Akbari,3 Sima Afsharnezhad,4 Parsa Farrokhi,5
    1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
    2. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    4. Department of biochemistry , Mashhad Branch, Islamic Azad University, Mashhad, Iran
    5. Faculty of medicine, Ilam University of Medical Sciences, Ilam, Iran


  • Introduction: FGF produced by endothelial cells induces proliferation and increases the survival of endothelial cells. FGF are collagenase activators and lead to the sprouting of new vessels from blood vessels, FGF is needed to maintain and progress angiogenesis. It is believed that VEGF acts in the beginning of angiogenesis. Doxycycline antibiotic has a wide biomedical application compared to other antibiotics. In laboratory conditions, it was found that this substance can inhibit endothelial growth and reduce tumor growth. . This study was conducted with the aim of investigating the effect of doxycycline antibiotic on the expression of FGF and VEGF genes in chicken embryos.
  • Methods: In this research, 40 Ross spray eggs were randomly divided into 5 groups including control, laboratory control (pbs) and 3 experimental groups. After incubation, on the second day of the window, an egg was created and in on the eighth day, after placing the gelatin sponge on the chorioalantoic curve, doxycycline antibiotic was injected with doses (50, 100 and 250 micromole/ milliliter) on the chorioallantoic membrane of chick embryos. In order to extract RNA and examine gene expression, a sample was taken from the chorioallantoic membrane and by making cDNA, the expression changes of VEGF and FGF genes were quantitatively measured. The collected data were analyzed by Excel and SPSS 20 statistical software.
  • Results: The average expression of VEGF and FGF genes in the laboratory control group did not show any significant difference compared to the control group. The average expression of VEGF and FGF genes in concentrations of 50, 100, 250 micromole/ml of doxycycline antibiotic showed a significant decrease compared to the control group.
  • Conclusion: According to this study, the doxycycline antibiotic has an inhibitory effect on angiogenesis in the chorioallantoic membrane of chick embryos. Also, it seems that the antibiotic doxycycline can be used to inhibit angiogenesis in cancer tissues.
  • Keywords: gene expression, Doxycycline antibiotic, Chorioalantoic Membrane, VEGF and FGF.