Model of neuropathic pain in Drosophila and Drosophila larvae using cisplatin
Model of neuropathic pain in Drosophila and Drosophila larvae using cisplatin
Mohammad sorush Ansari,1,*DR.Masoud Fereidoni,2
1. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran 2. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
Introduction: Neuropathic pain, a debilitating condition affecting up to 10% of the population, significantly impacts quality of life, contributing to stress, anxiety, depression, and disruption of daily routines. This type of pain arises from various factors, including diabetes, radiculopathy, nerve damage, and the use of anticancer drugs. Notably, chemotherapy-induced neuropathic pain, particularly associated with platinum-based agents like cisplatin, poses a significant clinical challenge. While cisplatin remains a crucial therapeutic option for various cancers, its use is often limited by the development of neuropathic pain. The precise mechanism underlying cisplatin-induced neuropathic pain remains elusive, highlighting the urgent need for further research. To facilitate such investigations, the establishment of an animal model utilizing cisplatin is essential to unravel the underlying mechanisms and explore potential therapeutic interventions.
Methods: In this study, wild-type Drosophila melanogaster and Drosophila third instar larvae were used to create a neuropathic model due to their physiological similarity to humans. Concentrations of 100 mg/L and 200 mg/L of cisplatin were added to the Drosophila culture medium, and after 6 days of feeding, the flies were isolated from the culture medium for the Hotplate test, and the duration of the flies' establishment on the hot plate It was recorded at temperatures of 41 to 50 degrees Celsius. (n=10) for Drosophila larvae, the larvae were placed on 3 cc of physiological serum on a hot plate, and the number of their movements during one minute was The temperature was observed and recorded from 35 to 41 degrees (n=10).
Results: One-way analysis of variance was performed using Graphpad prism10 software. The cisplatin group with a concentration of 100 mg/liter was not significantly different from the control group. (pANOVA>0.05) The 200 mg/liter cisplatin group had a significant difference with the control group and the cisplatin group with a concentration of 100 mg/liter. (PANOVA< 0.05). In Drosophila larvae, there was no significant difference between the 100 mg/L cisplatin group and the larval control group at any of the tested temperatures. (pANOVA>0.05) The larvae of 200 mg/L cisplatin group were significantly different from the control group at temperatures of 38 and 41 degrees. Being on the hot plate died.
Conclusion: The results show that 100 mg/L cisplatin does not cause neuropathic pain in adult Drosophila and its larvae, and 200 mg/L cisplatin causes neuropathic pain and hyperalgesia at all tested temperatures in larvae and Drosophila matures.