CRISPR/Cas9 genome editing approaches in cancer research
CRISPR/Cas9 genome editing approaches in cancer research
Hamed Esmaeil Lashgarian,1Hamidreza Khodadadi,2Leila Abkhooie,3Masumeh Jalalvand,4,*Amirmasoud Jalalvand,5
1. Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 2. Hepatitis Research Center, Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 3. Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 4. Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 5. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
Introduction: In cancer research, the CRISPR/Cas9 technology, with its notable genome engineering accuracy, has displayed effectiveness as a powerful method. CRISPR technology's ability to target and change specific genes linked to cancer in oncogenes, tumor suppressor genes, and other related genes paves the way for cancer treatment and novel cancer research.
Methods: Scientific sites and sources such as Scopus, Google Scholar, and PubMed will be used to conduct this study. Also, the keywords CRISPR system, Genome editing, Cancer therapy, Preclinical studies, and Clinical trials will be used. Articles that are very old (before 2000) or do not have any of these keywords are excluded from the study.
Results: Recent studies in various tumor fields have explored potential uses of CRISPR/Cas9 for gene-level treatment, and the development of personalized and targeted CRISPR/Cas9 medicines could transform tumor treatment. Numerous preliminary tumor treatment studies have been conducted in relevant fields. CRISPR/Cas9 may treat gene-level tumors.
Conclusion: However, CRISPR's clinical implementation in cancer therapy is still in its initial stages. This review elucidates the CRISPR technology and its role in cancer research, both pre-clinical and clinical. This could deepen researchers' understanding of cancer's genetic agents and lead to more advanced therapies.