Prevalence of Helicobacter Pylori by Antibodies titer Using ELISA and Compared to Stool Antigen in Urmia, Iran
Prevalence of Helicobacter Pylori by Antibodies titer Using ELISA and Compared to Stool Antigen in Urmia, Iran
Aysan Sheikhbaglou,1,*Mohammadtagi Arshadi,2Fatemeh Khoshsolok,3Soleiman Moradi Darmanderik,4Amin Saadat Asqarkhani,5Farzin Samadi,6
1. Department of Microbiology and Parasitology, Kia Tashkhis Ayaz laboratory, Urmia, Iran 2. Department of Microbiology and Parasitology, Kia Tashkhis Ayaz laboratory, Urmia, Iran 3. Department of Microbiology and Parasitology, Kia Tashkhis Ayaz laboratory, Urmia, Iran 4. Department of Microbiology and Parasitology, Kia Tashkhis Ayaz laboratory, Urmia, Iran 5. Department of Microbiology and Parasitology, Kia Tashkhis Ayaz laboratory, Urmia, Iran 6. Department of Microbiology and Parasitology, Kia Tashkhis Ayaz laboratory, Urmia, Iran
Introduction: Helicobacter pylori (H. pylori) is one of the most common human infections in the world.(1) This bacterium is also associated with some cases of gastric mucosal lymphoma or maltoma and seems to be a predisposing factor for the development of gastric carcinoma .In developing countries, most children (80%) are infected by the age of 10, while in advanced countries, the prevalence of H. pylori and gastritis increases with age(2). The aim of this study was to evaluate the antibody titer of IgG and IgM anti-Helicobacter pylori using ELISA method and to investigateits relationship with stool antigen.
Methods: This study was conducted in 2024 on 100 patients refering to Dr. Nemati Laboratory in Urmia. After blood sampling and serum isolation, IgG and IgM antibody titers were measured by ELISA method. On the other hand, stool antigens were also tested using the Helicobacter pylori Rapid Kit.
Results: Results showed that 58% of IgG positive and 65% of H.pylori Ag stool positive were studied. Other results showed that 30% of patients were both IgG positive and H.pylori Ag stool positive. No association was found between H. pylori Ag stool and IgG with IgM. which means that IgM antibody was negative in all patients. That is, H.pylori Ag stool is the best and most accurate method for Helicobacter pylori screening.
Conclusion: Simple methods based on the search for antibodies and antigens with high sensitivity and specificity are available. The advantage of ELISA in addition to its high sensitivity and specificity also determines the antibody class. IgG has a higher diagnostic value and prognosis and represents a chronic stage of the disease.IgM is not very useful in the diagnosis of Helicobacter pylori and can be said to be an insensitive indicator of acute infection. On the other hand, for detection of Helicobacter pylori antigen in stool rapids can also be very useful due to its high accuracy and low test time.