Antimicrobial Effect of Extract Equisetum arvense and Its Toxicity on lung Cancer SKmes1 Cells Line
Antimicrobial Effect of Extract Equisetum arvense and Its Toxicity on lung Cancer SKmes1 Cells Line
Sama Delfanian,1,*
1. M.sc student of Pathogenic Microbes Islamic Azad University Mazandaran,Tonekabon,Iran
Introduction: Lung cancer is one of the most common cancers and chemotherapy is one of the cancer treatment methods, but due to the lack of selective cytotoxicity, it leads to unbearable side effects. Also, due to the increase in resistance of bacteria to antibiotics and the presence of antimicrobial compounds in plants, it has been taken into consideration. Therefore, the purpose of this research is to determine the antimicrobial activity of aerial part and root extracts of the Equisetum arvense and their inhibitory effect on the growth of SKmes1 lung cancer cells.
Methods: For this purpose, after collecting Equisetum arvense plant, extraction was done and Then the Antimicrobial activity of the extracts on pathogenic bacteria was measured by disk diffusion, MIC and MBC methods. Further, after the culture and proliferation of SKmes1 cell line, the cells exposed to different concentrations of aerial part and root extracts of the Equisetum arvense plant and were incubated for 24, 48 and 72 hours. Then, the MTT colorimetric test method was used to determine the cytotoxicity of the extract.
Results: The results have shown that Equisetum arvense L. exhibited antibacterial effects only on pathogenic gram-positive cocci.
Another study showed that they investigated the inhibitory effect of hydro-alcoholic extract of Thymbra spicata on the growth of SKmes1 lung cancer cell line. The results of the MTT test showed that this extract has a dose-dependent cytotoxic effect on SKmes1 cells. The findings indicated that the extract of mountain thyme has an inhibitory effect on lung cancer cells due to the presence of phenolic compounds.
Conclusion: According to the obtained results from the study, E. arvense extract can be used as herbal medicine for cancer; however, further research especially in vivo evaluation should be carried out to evaluate the therapeutic potential of E. arvense against cancer. Leaf extracts generally showed higher antibacterial activity than stem extracts.
In addition, the highest antibacterial activity was found in the methanolic leaf extract. As a result, horsetail is not only healthy food but also helpful in protecting from various diseases due to its antibacterial activity. We believe it will bring a high added value to the scientific world by examining the effects of the bioactive components on various diseases in detail in future studies.