Evaluation of synergistic effects of selected combination therapy in comparison with mesalazine on characteristics of ulcerative colitis experimental model
Evaluation of synergistic effects of selected combination therapy in comparison with mesalazine on characteristics of ulcerative colitis experimental model
Mostafa Eslamimahmoudabadi,1Hadi Esmaeili Gouvarchin Ghaleh,2,*
1. Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran 2. Applied Virology Research Center, Baqiyatallah University of medical sciences, Tehran, Iran.
Introduction: Individuals with Crohn's disease and ulcerative colitis suffer from chronic intestinal diseases known as "inflammatory bowel diseases," which are costly to treat. Despite currently prescribed medications, there are still adverse effects that must be reduced or avoided altogether. Therefore, the use of natural supplements may be useful in order to reduce the drug dose and the symptoms of the disease.
Goal: The purpose of the present study is to investigate the synergistic effects of combined treatment of selected probiotics, vitamin D, pomegranate extract, propolis, and curcumin compared to mesalazine on the clinical and immunological features of ulcerative colitis model mice.
Methods: Nine groups of mice were administered either a single treatment or a combination of agents after the animal model was induced with acetic acid. After a month, samples were taken, and colorimetric techniques were used to assess the expression levels of inflammatory cytokines (IL-1, IL-6, and TNF-α) and oxidative agents (Myeloperoxidase and nitric oxide). The Disease activity index (DAI) and histopathological condition were also considered after mice scarification. MTT assay was carried out to assess cell growth.
Results: Our results demonstrated that combination therapy reduced DAI, nitric oxide, and Myeloperoxidase activity, proliferation, and inflammatory cytokines in the treated groups. Additionally, these treated groups also experienced a decrease in pathology scores. Except when the combination of treatments was administered, these declines were not significant. As demonstrated in this study, a combination of anti-inflammatory agents had a suppressive effect comparable to mesalazine.
Conclusion: By administration combining lactobacillus casei, VIT D, propolis, pomegranate, and curcumin in induced-ulcerative colitis mice, oxidative and inflammatory parameters were alleviated. Result similar to mesalazine were also obtained with this treatment.