The seminal plasma exosomes concentration as a potential marker in Varicocele men
The seminal plasma exosomes concentration as a potential marker in Varicocele men
Nasim Mohammadi,1,*Rahil Jannatifar,2Nasim Hayati Roodbari,3
1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2. Department of Reproductive Biology, Academic Center for Education, Culture and Research (ACECR), Qom, 3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Introduction: Background: Varicocele-associated stressors, such as hypoxia and heat, can damage cell function and viability, and some exosomal biomarkers released from impaired cells may reflect the cell status in testis. The purposes of this study investigate the seminal plasma exosomes on sperm function sperm function in varicocele patients.
Methods: Methods: Normozoospermic, and varicocele men (men25–40years of age) were considered for the study. Seminal plasma was collected and processed to separate spermatozoa and exosomes. Exosome uptake by spermatozoa was monitored by means of Bradford and flow cytometry. The effect of exosomes on spermatozoa was determined by evaluating sperm parameters According to the World Health Organization criteria (WHO) (2010).
Results: Results: We isolated and characterized exosomes from seminal plasma of Normozoospermic, varicocele patients. Exosomes concentration derived from varicocele significantly was decreased than normozoospermic individuals (p < 0.05). The current study showed a statistically significant highly positive correlation in Exosomes concentration with sperm count, motility, normal morphology, and viability. However, it shows significant negative correlation with sperm DNA fragmentation (p < 0.05).
Conclusion: Conclusions: These findings provide evidence that exosomes in seminal plasma can effect on sperm quality. In the future, Seminal exosome may be a novel, sensitive, and non-invasive biomarker of Sertoli cell damage in Varicocele.