Introduction: Replacing damaged organs or tissues and repairing damage by tissue engineering are attracting great interest today.
Today, electrospun PCL-based scaffolds are widely used for tissue engineering applications. In this study, we used electrospun polycaprolactone (PCL) scaffold coated with fibronectin (Fn), a ubiquitous ECM glycoprotein, to investigate the induction potential of this scaffold in osteogenesis with adipose-derived mesenchymal stem cells (AD-MSCs).
The results also showed that alkaline phosphatase activity was significantly higher in the PCL scaffold coated with Fn than in the control groups (PCL scaffold group and tissue culture polystyrene (TCPS) group) .
Methods: Preparation of scaffolding
Scaffold hydrophilicity and Morphological Properties
Cell Culture
The ability of AD-MSCs differentiation
Oil red staining
Alizarin red staining
Cell Viability Assay
Alkaline Phosphatase Activity
Evaluation of osteogenic differentiation of AD-MSCs
Results: Scaffold Characterization
The microstructure of the PCL coated with fibronectin was evaluated by SEM. The results showed that fibronectin increased the hydrophilic and biocompatible properties of the electrospun PCL scaffold
Conclusion: The results of this study have shown that PCL modified with fibronectin has excellent osteogenic induction potential by increasing the expression of bone marker genes, the activity of ALP,,and calcium deposition. Therefore, it has promising potential for use in bone tissue engineering.
Keywords: Bone Tissue Engineering, Mesenchymal Stem Cells, PCL, Fibronectin