The Effect of nanomicelle Curcumin on Post-Thaw Quality of Cryopreserved Human Sperm
The Effect of nanomicelle Curcumin on Post-Thaw Quality of Cryopreserved Human Sperm
Amir Rabiei,1,*Mojgan Saghazadeh,2Rahil Jannatifar,3
1. Department of Genetics, Qom Branch, Islamic Azad University, Qom, Iran. 2. Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran 3. Department of Reproductive Biology, Academic Center for Education, Culture and Research (ACECR), Qom, Iran.
Introduction: Human sperm cryopreservation is an important tool for assisted reproductive technology and male fertility preservation. The antioxidant effects of curcumin on different cells have been widely reported. This study was aimed to evaluate the potential role if the effect of nanomicelle Curcumin supplementation on sperm quality, and DNA integrity.
Methods: Semen of 25 oligoasthenoteratozoospermia men was collected and each sample was divided into three equal aliquots: Control, nanomicelle Curcumin. The samples were analyzed freshly for viability (Eosin Y), morphology (Diff-Quick), motility (following WHO standards), DNA integrity (SCD Test). The control group remained untreated and was mixed with cryopreservation medium (1:1). The nanomicelle Curcumin group was mixed with cryopreservation medium containing 10 μM nanomicelle Curcumin. Ten days after cryopreservation, samples were thawed and pre-freeze analyses repeated.
Results: Obtained results showed that cryopreservation significantly (P < 0.001) reduces sperm parameters. In nanomicelle Curcumin group, viability, morphology, motility, and DNA integrity significantly (P < 0.001) increased after the thawing process, as compared with the control group.
Conclusion: These results suggest that the addition of nanomicelle Curcumin to cryopreservation medium improves post-thaw sperm quality, sperm DNA integrity. Further research is needed on the use of nanomicelle Curcumin, and other antioxidant substances in sperm cryopreservation.