The study of the Modulation of collagen alignment by stem cells in mechanical properties of wound healing
The study of the Modulation of collagen alignment by stem cells in mechanical properties of wound healing
Farzaneh Chehelcheraghi,1,*
1. Farzaneh Chehelcheraghi Medical Ethics and Law Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Introduction: Recently, the use of random flaps has become widespread in the field of plastic surgery. With the clinical application of random flaps in plastic surgery, skin defects can be improved in terms of aesthetics and functionality. With the development of regenerative medicine, stem cell therapy is a promising way to increase regeneration and accelerate tissue healing in chronic wounds. Our objective in this study was to determine the effect of bone marrow stem cells on the modulation of collagen formation in improving the mechanical properties of wound healing in male albino Wistar rats.
Methods: Twenty male albino Wistar rats with an average weight of 250-300g were used in this research. Also, five rats of the same breed weighing 30 to 40 grams were used to extract bone marrow cells. The animals were divided into two healthy groups without therapeutic intervention and with therapeutic intervention of stem cell injection so there were ten animals in each group. In both groups, day zero was the day of surgery and all the 20 animals underwent surgery. A random skin flap measuring 3 x 8 cm was created in the animals' back area, and the results were checked on the 14th day in the transitional area.
Results: BMMSC injection increased the survival level and decreased the necrotic wound level, but this improvement was not statistically significant. Also, the infusion of BMMSCs improved the biomechanical properties of wound healing, the increase of which was statistically significant except for energy absorption. However, in terms of the synthesis, content, and arrangement of collagen fibrils, the injection of BMMSCs was ineffective. In the untreated group, there was more collagen and a more organized arrangement in collagen fibrils.
Conclusion: Local injection of BMMSCs did not significantly increase the survival level of random skin flap but improved the wound healing process. Also, the infusion of BMMSCs did not increase collagen content and improve the arrangement of collagen fibrils but significantly improved the biomechanical properties of the wound