Fabrication and optimization of co-administrated Elaeagnus Angustifolia extract and Rosa canina isolated polysaccharide-loaded electrospun gelatin/polycaprolactone nanofibers as the burn wound dressing
Fabrication and optimization of co-administrated Elaeagnus Angustifolia extract and Rosa canina isolated polysaccharide-loaded electrospun gelatin/polycaprolactone nanofibers as the burn wound dressing
Soraya Sajadimajd,1,*Maryam Nazari,2Gholamreza Bahrami,3Bahareh Mohammadi,4
1. Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran 2. Applied Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran 3. Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran 4. Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran
Introduction: Modified wound dressings by providing a regenerative and anti-bacterial environment can accelerate the healing of wounds and prevent infection. This study aims to fabricate and evaluate the healing effect of an electrospun wound dressing containing polycaprolactone (PCL), gelatin (Gel), and extracts of Elaeagnus Angustifolia and isolated polysaccharide from Rosa canina on a burn wound model.
Methods: Characteristics analyses including scanning electron microscopy (SEM), FT-IR spectroscopy (FTIR), and X-ray diffraction (XRD) were performed to survey the physical and chemical properties of PCL/Gel/Polysaccharide and PCL/Gel/Extract mats. Water contact, mechanical properties, control release, cellular behavior, and microbial penetration were investigated to optimize dressings. The cytoprotective effect of wound dressing was evaluated on fibroblast cells. The rat burn model was used to examine the accelerative healing potentials of scaffolds.
Results: PCL/Gel mats containing Rosa canina polysaccharide or Elaeagnus Angustifolia extract as the well-known therapeutic agents showed that they can reduce pain, stimulate re-epithelialization, induce vascular propagation, and reduce infection.
Conclusion: The designed wound mat seems to be a novel mat to heal several wounds compared to others.