development of nanofibrous scaffold incorporated with ZnO NPs against human melanoma A357 cells
development of nanofibrous scaffold incorporated with ZnO NPs against human melanoma A357 cells
vida vahdanikia,1Abolfazl Barzegar,2Mehdi Haggi,3Somayeh Ebrahimzadeh,4,*
1. Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran 2. Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran 3. Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran 4. Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Introduction: Skin cancers represent the most prevalent form of malignancy among humans. The exploration and identification of potent pharmaceuticals for skin cancer have emerged as a critical objective, given the pervasive and perilous proliferation of this malignancy globally. Consequently, it is essential to conduct thorough research on this disease and to investigate the nanomaterials that influence it, with the aim of developing effective therapeutic strategies to eradicate this condition. Therefore, this research investigated the impact of polycaprolactone collagen nanopads embedded with zinc oxide nanoparticles on human melanoma A375 cells in vitro.
Methods: The synthesis of polycaprolactone/collagen nanofibers (NFs-PCL-Coll) integrated with zinc oxide nanoparticles (ZnO NPs) was accomplished through the electrospinning technique. Nanoparticles were produced through a green synthesis method utilizing orange peel and then the characteristics of the resulting nanoparticles and nanofibers were examined. The assessment of the synthesized nanopads' toxicity on the A357 cell line was conducted through the MTT assay. Upon achieving the IC50 concentration, the A357 cell line was subjected to treatment at this concentration, followed by an analysis of cell death rates and morphological changes.
Results: The analysis of the MTT assay results revealed a marked decrease in cell viability when exposed to the IC50 concentration of zinc oxide-containing nanofibers. Furthermore, morphological assessments indicated that the treatment group exhibited a higher prevalence of deformed cells than the control group.
Conclusion: The findings presented above suggest that ZnO-NPs/PCL-Coll, as an unconventional material in cancer therapy, demonstrate promising potential for effectively treating and eradicating skin cancer