• Chitosan/ Egg Shell Membrane Hydrogel as a Novel Biocompatible Composite with Antibiofilm Activity
  • zahra parandeh,1 Faezeh Takhsha,2 Zahra Sedighi,3 Arefe Sadat Khavari,4 Sana Razzazi,5 Fatemeh Sadat Shariati,6,*
    1. Department of Biotechnology , school of Advanced Technologist in Medicine , Shahid Beheshti university of Medical Sciences , Tehran , Iran
    2. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    3. department of Medical Biotechnology , Faculty of Medicine, Shahed University, Tehran , Iran
    4. Department of Biology , College of Basic Sciences , Shahed university , Tehran , Iran
    5. Department of Biology , Faculty of Basic Siences , Shahed University , Tehran , Iran
    6. Infulenza Research Lab , Pasteur Institute of Iran , Tehran , Iran


  • Introduction: Novel biocompatible and anti-biofilm chitosan based hydrogel was synthesized and embedded with waste Egg Shell Membrane powder. The functional agents in each synthesis step had been confirmed using Fourier-transform infrared (FT-IR). Field-emission scanning electron microscope (FE-SEM) revealed a highly porous composite with a three-dimensional mesh structure. Different methods were used to explore the biological capability of the hydrogel. Results showed that prepared hydrogel prevents the formation of Staphylococcus aureus biofilm. The hemolytic assay demonstrated that almost all red blood cells survived. The cell viability on mouse fibroblast cells was 94.3% after an overnight incubation whereas it was about 96% for the control group. The statistical analysis indicated that there was no remarkable difference between the cell viability of the control and treated groups (P-value > 0.05). In conclusion, the fabricated Chitosan/ Egg Shell Membrane hydrogel showed proper properties that candidate it as a safe anti-biofilm substance in various medical materials.
  • Methods: A novel biocompatible and anti-biofilm chitosan-based hydrogel was synthesized and embedded with waste egg shell membrane powder. The functional agents in each synthesis step had been confirmed using Fourier-transform infrared (FT-IR). Field-emission scanning electron microscope (FE-SEM) revealed a highly porous composite with a three-dimensional mesh structure. Different methods were used to explore the biological capability of the hydrogel.
  • Results: The results showed that the prepared hydrogel prevents the formation of Staphylococcus aureus biofilm. The hemolytic assay demonstrated that almost all red blood cells survived. The cell viability on mouse fibroblast cells was 94.3% after an overnight incubation, whereas it was about 96% for the control group. The statistical analysis indicated that there was no remarkable difference between the cell viability of the control and treated groups (P-value > 0.05).
  • Conclusion: In conclusion, the fabricated chitosan/egg shell membrane hydrogel showed proper properties that position it as a safe anti-biofilm substance in various medical materials.
  • Keywords: Chitosan, Egg Shell Membrane, Staphylococcus aureus, Biofilm