• DCs Pulsed with Hypochlorous Acid-Treated Tumor Cell Lysates present antigens efficiently and induce CD8+ T cell activation through cross-presentation
  • Maryam Abbaspour,1 Vajihe Akbari,2,* Nafiseh Esmaeil,3
    1. Isfahan University of Medical Sciences
    2. Isfahan University of Medical Sciences
    3. Isfahan University of Medical Sciences


  • Introduction: In the process of initiating and regulating immune responses, dendritic cells (DCs) play an important role as antigen-presenting cells. When dendritic cells are exposed to tumor cell lysates , they can stimulate T cells to recognize tumor-associated and tumor-specific antigens and generate an immune response against cancer. The purpose of this study, was to compare four different approaches for preparing breast tumor cell lysates for pulsing DCs.
  • Methods: To prepare tumor cell lysates from 4T1 cells, four different methods were used, including freeze-thaw (FT), hypochlorous acid (HOCl), hyperthermia (HSP), and UVB irradiation (UV). Using flow cytometry and ELISA, we assessed the effects of these tumor lysates on DC maturation and cytokines secretion. Furthermore, DCs pulsed with different lysates were also evaluated for their ability to promote CD8+T cell proliferation and release cytokines.
  • Results: Our data demonstrate that DCs pulsed with lysate prepared by HOCl pulsed with HOCl exhibited more maturation surface biomarker expression (CD86) compared to DCs pulsed with freeze-thawed cells or unloaded DCs (control) (P<0.0.5). Furthermore, activated DCs were also found to promote CD8+T cell proliferation and induce T cells responses by producing high levels of IFN-γ, while inhibiting IL-10
  • Conclusion: HOCl is capable of releasing tumor antigens while maintaining their ability to stimulate an immune response. .DC-based therapies may be designed based on the findings presented here, demonstrating a cross-presentation of antigens and specific activation of the immune system against breast cancer.
  • Keywords: Breast cancer; Dendritic cells; Tumor cell lysate; Hypochlorous acid