• Investigating the effect of Baneh tree leaf extract on acne-causing bacteria (Propionibacterium acnes(
  • sara maghsodiannejad,1,* Mehran Aalivand,2 yekta Alimohamadi,3 Taranom dezhagah,4 Niayesh yosefi,5 Sorya Orak,6
    1. Izeh Hashabi Doctor's Research Center/.Izeh, khozestan, Iran
    2. Izeh Hashabi Doctor's Research Center.Izeh, khozestan, Iran
    3. Farzangan Izeh High School/ Izeh, khozestan, Iran.
    4. Farzangan Izeh High School/ Izeh, khozestan, Iran
    5. Farzangan Izeh High School/ Izeh, khozestan, Iran
    6. Farzangan Izeh High School/ Izeh, khozestan, Iran


  • Introduction: Nowadays, investigating the antimicrobial effects of plant extracts, especially plants that have traditional medicinal uses, is one of the topics of interest to researchers. Since ancient times, medicinal plants (due to having essential oils) have played an important role in human health According to the researches conducted on the antimicrobial effects of the extract of baneh leaves and also the information of the local people of Izeh city who used the concentrated aqueous extract of baneh leaves to prevent the infection of deep wounds, in this research the effect of aqueous and ethanolic extracts Bene leaves have been investigated on acne-causing bacteria to find a herbal and organic treatment against this widespread problem, bacterial acne disease.
  • Methods: First, the ethanolic and aqueous extracts of Beneh leaves were prepared by soaking. The extracts were prepared with three concentrations of 200 mg/ml, 400 mg/ml and 600 mg/ml. 2 ml of each of the ethanolic and aqueous extracts were taken separately and mixed with 20 ml of DMSO in separate vials. For control treatment, DMSO without extract was used. Then, the microbial solution, with McFarland's 0.5 standard, was inoculated into the Muller-Hinton agar culture medium after preparing from the original stock solution. After the incubation of different concentrations of the extracts and the penetration of the extracts into the culture medium, the diameter of the growth halo (in millimeters) was measured.
  • Results: The results of the effect of the concentrations of ethanolic and aqueous extracts of Beneh tree leaves by the well diffusion method are shown in Table (2). Statistical investigations and conducting t-test and examining P Values (with 95% confidence) of each group revealed that the effectiveness of both aqueous and ethanolic extracts was statistically significant and the respective P Values were 0.045 and 0.040, respectively. In this way, the value of p, the water extract of Beneh plant was found to be p value˂0. 05 was smaller and it is statistically significant. These results are consistent with the initial averages.
  • Conclusion: The results of our research in the study of the non-growth halo of the aqueous extract of the pistil leaves of the Pistacia genus on the propioni bacteria strain have shown that different concentrations of the aqueous and ethanolic extracts of the pistil leaves had an inhibitory effect on the propioni bacteria, although the diameter of the non-growth halo was different in different dilutions. has been Also, the effect of the aqueous extract of the tuber on inhibiting the growth of bacteria was greater than the ethanolic extract, which was consistent with the way the local people used the extract boiled with water to prevent infection of deep wounds. investigated the effect of walnut leaf extract on Propionibacterium acnes and reported the inhibitory effect of this extract. Regarding the effect of baneh leaf extract on other microorganisms, researches have been conducted that have all shown the inhibitory effect of this extract on the growth of bacteria, including Mahdavi et al. They investigated Staphylococcus, Escherichia coli and Pseudomonas microbes and reported the greatest inhibitory effect of Beneh leaf extract on Pseudomonas microbes. In a research conducted by Benhammou et al.,
  • Keywords: agar, antimicrobial, tuber extract, medicinal value.