Investigation of surface modification on the size and zeta potential of PLGA nanoparticles
Investigation of surface modification on the size and zeta potential of PLGA nanoparticles
Maral Motamedi,1,*Fatemeh Madani,2Masood Khosravani,3Mahdi Adabi,4
1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences 2. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences 3. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences 4. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences
Introduction: There is a huge emergence using nanoparticles in novel drug delivery platforms and polymeric nanoparticles are the second most popular type of nanostructures. PLGA (poly(lactic-co-glycolic acid)) is a kind of highly studied synthetic polymer because of its biodegradability, biocompatibility, and extended drug release. Besides the nature of polymeric structures, their surface, size, and zeta potential play important roles in the efficient delivery of nano-carriers and their bio-fate . In this study, we aimed to tune the surface of PLGA nanoparticles with substances such as PVA, poloxamer188, poloxamer407, tween20, and tween80 at different ratios and investigate the nanoparticle's mean diameter and zeta potential.
Methods: Nanoparticles were prepared using precipitation and single-emulsion techniques. 30 mg of PLGA was dissolved in 3 ml of acetone or dichloromethane and added to the 30 ml water-based phase. The size and zeta potential of nanoparticles were analyzed by DLS, SEM, and zeta analyzer, respectively.
Results: The mean diameter and zeta potential of Tween80 and tween20 coated nanoparticles were varied from 225-300 nm, 170-610 nm, and -10 to -21 mV, and -13 to -18 mV, respectively. Poloxamer188 and poloxamer407 coated nanoparticles had mean sizes of 230-401 nm and 148-358 nm, respectively. Moreover, their zeta potential varied from -12 to -19 mV and -12 to -16 mV, respectively.
Conclusion: According to the results of our study, it was observed that there is an optimum concentration of surfactants for achieving superior size and zeta potential of nanoparticles.