CRISPR-Mediated Modulation of mRNA Expression in Breast Cancer Cells: Implications for Targeted Therapy
CRISPR-Mediated Modulation of mRNA Expression in Breast Cancer Cells: Implications for Targeted Therapy
Kosar Sobhani,1,*
1. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz. Iran
Introduction: Breast cancer remains a significant global health concern, necessitating the exploration of innovative therapeutic strategies. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology offers precise control over mRNA expression levels, presenting a promising avenue for targeted therapy. This study investigates the efficacy of CRISPR-based mRNA modulation in breast cancer cells, focusing on specific genetic targets and therapeutic implications.
Methods: Patient-derived breast cancer cell lines representing different subtypes were subjected to CRISPR-mediated mRNA modulation targeting key oncogenes or tumor suppressor genes relevant to breast cancer pathogenesis. mRNA expression levels were quantified using quantitative real-time PCR before and after CRISPR-mediated modulation. Functional assays, including cell proliferation, migration, and apoptosis assays, were performed to assess the impact of mRNA modulation on breast cancer cell behavior.
Results: CRISPR-mediated mRNA modulation effectively altered the expression levels of targeted genes in breast cancer cells. Downregulation of oncogene mRNA expression, such as HER2 (mean mRNA copies: 1000 to 200; p<0.001), resulted in decreased proliferation and migration, while upregulation of tumor suppressor mRNA expression, such as BRCA1 (mean mRNA copies: 200 to 800; p<0.001), led to enhanced apoptosis. These findings demonstrate the functional significance of CRISPR-mediated mRNA modulation in modulating breast cancer cell behavior.
Conclusion: The study highlights the therapeutic potential of CRISPR-based mRNA modulation as a targeted approach in breast cancer therapy. By precisely controlling gene expression levels, CRISPR technology offers a tailored strategy for disrupting oncogenic pathways and enhancing tumor suppressor functions specific to breast cancer. This research underscores the role of CRISPR-based mRNA modulation in advancing precision oncology interventions for breast cancer treatment.