A study on the interaction of angiotensin i converting enzyme inhibitory peptide with human hemoglobin protein by conductometry method in the absence and presence of a low frequency electromagnetic fi

Farzaneh Sadeghzadeh,1,* Jamshidkhan chamani1*,2 Farzin hadizadeh2,3

Abstract

Introduction

High blood pressure, or hypertension, is a chronic disease in which the blood pressure goes up. angiotensin converting enzyme plays a major role in increasing blood pressure, and one of the over-stressed therapeutic goals is to inhibit this enzyme. angiotensin converting enzyme inhibitors help loosen and expand blood vessels. the purpose of this study is to investigate electromagnetic fields effects with low frequencies (10 hz, 1 khz and 100 khz) on the interaction between a peptide inhibitor of angiotensin enzyme and human hemoglobin by conductometry method because hemoglobin is able to bind to many internal and external factors, including many drugs, platinum drugs, anti-tumors, and many chemical compounds.

Methods

Hb with molecular mass of 64500 dalton, angiotensin i converting enzyme inhibitory peptide with molecular mass of 1582.74 dalton, phosphate potassium buffer. the conductometry was performed by metrohm, that used for measuring electric conductivity of the solution.

Results

Studies indicated that with increasing concentration of peptide, electrical conductivity has increased. in addition, in the presence of magnetic fields, ionizing groups can be created. increasing ionized groups causes to increase the solute electrical conductivity. it can be argued that, with increasing electromagnetic field, the conductometry has increased.

Conclusion

The interaction between the hemoglobin protein and the hypotensive drug has taken place and the presence of a low-frequency electromagnetic field is agent for a strong bond between the protein and the peptide.

Keywords

Human hemoglobin, angiotensin i converting enzyme inhibitory peptide, conductometry