Frequency of binding site at c-terminal of helicobacter pylori caga separated from gastric tissue biopsy of patients with gastroduodenal disorders in shafa hospital in khorramabad
Razieh Peykarestan,
1,* Mohsen mirzaei,
2
1. Master's Degree, Department of Microbiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
2. Department of Laboratory sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Abstract
Introduction
helicobacter pylori (h. pylori) is a gram-negative, microaerophilic bacterium in gastric mucosa. it has a helical shape and is urease, catalase, and oxidase positive. the caga gene is a h. pylori virulence factor. it is present downstream of an approximately 40-kb-long cag pathogenicity island (cagpai), which encodes the 120–145 kda caga protein. investigation into frequency of binding site at c-terminal of caga separated from gastric tissue biopsy of patients infected with h. pylori.
Methods
dna extraction was done using dng plus kit (cinaclon company) on biopsy samples of infected patients, who were positive for rut. to perform polymerase chain reaction (pcr) to investigate the presence of 16srrna, which confirms positive results for rut, and also to investigate binding form and frequency of caga in dnas extracted from biopsy samples, a pair of primer was selected and subjected to molecular examination to investigate the c-terminal region of caga and one pair to investigate 16srrna gene.
Results
all biopsy samples were infected with 16srrna. in addition, the frequency and binding form of this gene, regarding the designed primer from c-terminal region, was measured as 49% in 100 samples.
Conclusion
The frequency of this pathogenic gene among patients with gastrointestinal symptoms, visiting the hospital, easy transfer of this bacterium among people, and changes and diseases developed based on binding form of caga in host cells provoke an alarm about an outbreak of lethal cancers among people in future.
Keywords
helicobacter pylori, caga،c-terminal, polymerase chain reaction
